PMID- 15757528
OWN - NLM
STAT- MEDLINE
DCOM- 20060705
LR  - 20150313
VI  - 24
IP  - 3
DP  - 2005 Mar
TI  - [Inducing specific antitumor effects on osteosarcoma by fused vaccine of rat 
      dendritic cells].
PG  - 285-91
AB  - BACKGROUND & OBJECTIVE: Dendritic cells (DCs) are the strongest antigen 
      presenting cells (APCs). But immune therapy based on DCs for osteosarcoma has 
      seldom been reported. This study was designed to investigate the immunologic 
      potency and antitumor effects of fused vaccine of rat DCs on osteosarcoma. 
      METHODS: Mononuclear cells in rat bone marrow were propagated in vitro under the 
      condition of recombinant granulocytemacrophage colony-stimulating factor 
      (rGM-CSF), recombinant interleukin-4 (rIL-4), and recombinant tumor necrosing 
      factor-alpha (rTNF-alpha) to obtain DCs. DCs were purified by monoclonal antibody 
      OX62 and magnetic beads, and confirmed by morphologic observation, phenotype 
      determine, and functional experiments. Osteosarcoma cell line UMR-106 was fused 
      with allogeneic or syngeneic DCs by electrofusion to produce the tumor vaccine. 
      Each kind of tumor vaccine was injected subcutaneously into 10 rats to observe 
      its specific antitumor effects. RESULTS: Survival rate of rats after injection of 
      1x10(7) UMR-106 cells was 70% in allogeneic tumor vaccine group, and 50% in 
      syngeneic tumor vaccine group. The survival rats stood up to the second challenge 
      of 1x10(7) UMR-106 cells 7 weeks after injection. After immunization of 2x10(6) 
      UMR106-DCs, tumors in 60% of the rats bearing pre-established tumor cells 
      diminished or vanished. CONCLUSION: Fused tumor vaccine of UMR-106 cells and 
      allogeneic DCs might induce specific anti-tumor effects on osteosarcoma.
FAU - Yu, Zhe
AU  - Yu Z
AD  - Center of Orthopedic Surgery, Orthopedics Oncology Institute of Chinese PLA, 
      Tangdu Hospital, The Fourth Military Medical University, Xi'an, Shaanxi, 710038, 
      P.R.China. yuzhe19@fmmu.edu.cn
FAU - Fan, Qing-Yu
AU  - Fan QY
FAU - Ma, Bao-An
AU  - Ma BA
FAU - Hao, Xin-Bao
AU  - Hao XB
FAU - Long, Hua
AU  - Long H
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Ai Zheng
JT  - Ai zheng = Aizheng = Chinese journal of cancer
JID - 9424852
RN  - 0 (Cancer Vaccines)
SB  - IM
MH  - Animals
MH  - Bone Neoplasms/*immunology/pathology/therapy
MH  - Cancer Vaccines/*immunology
MH  - Cell Fusion
MH  - Cell Line, Tumor
MH  - Cells, Cultured
MH  - Dendritic Cells/cytology/*immunology
MH  - Male
MH  - Osteosarcoma/*immunology/pathology/therapy
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Rats, Wistar
EDAT- 2005/03/11 09:00
MHDA- 2006/07/06 09:00
CRDT- 2005/03/11 09:00
PHST- 2005/03/11 09:00 [pubmed]
PHST- 2006/07/06 09:00 [medline]
PHST- 2005/03/11 09:00 [entrez]
AID - 1000467X2005030285 [pii]
PST - ppublish
SO  - Ai Zheng. 2005 Mar;24(3):285-91.