PMID- 15757647
OWN - NLM
STAT- MEDLINE
DCOM- 20050425
LR  - 20071119
IS  - 0014-5793 (Print)
IS  - 0014-5793 (Linking)
VI  - 579
IP  - 7
DP  - 2005 Mar 14
TI  - Inhibition of the angiogenesis by the MCP-1 (monocyte chemoattractant protein-1) 
      binding peptide.
PG  - 1597-601
AB  - The CC chemokine, monocyte chemoattractant protein-1 (MCP-1), plays a crucial 
      role in the initiation of atherosclerosis and has direct effects that promote 
      angiogenesis. To develop a specific inhibitor for MCP-1-induced angiogenesis, we 
      performed in vitro selection employing phage display random peptide libraries. 
      Most of the selected peptides were found to be homologous to the second 
      extracellular loops of CCR2 and CCR3. We synthesized the peptide encoding the 
      homologous sequences of the receptors and tested its effect on the MCP-1 induced 
      angiogenesis. Surface plasmon resonance measurements demonstrated specific 
      binding of the peptide to MCP-1 but not to the other homologous protein, MCP-3. 
      Flow cytometry revealed that the peptide inhibited the MCP-1 binding to THP-1 
      monocytes. Moreover, CAM and rat aortic ring assays showed that the peptide 
      inhibited MCP-1 induced angiogenesis. Our observations indicate that the 
      MCP-1-binding peptide exerts its anti-angiogenic effect by interfering with the 
      interaction between MCP-1 and its receptor.
FAU - Kim, Mee Young
AU  - Kim MY
AD  - Department of Molecular Biology, Institute of Nanosensor and Biotechnology, 
      Dankook University, Hannam-dong san 8, Yongsan-ku, Seoul 140-714, Republic of 
      Korea.
FAU - Byeon, Cheol Woo
AU  - Byeon CW
FAU - Hong, Kyung Hee
AU  - Hong KH
FAU - Han, Ki Hoon
AU  - Han KH
FAU - Jeong, Sunjoo
AU  - Jeong S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Angiogenesis Inhibitors)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Oligopeptides)
RN  - 0 (Peptide Library)
RN  - 0 (Receptors, CCR2)
RN  - 0 (Receptors, Chemokine)
RN  - 0 
      (histidyl-seryl-tryptophyl-arginyl-histidyl-phenylalanyl-histidyl-threonyl-leucyl-glycyl-glycyl-glycine)
SB  - IM
MH  - Angiogenesis Inhibitors/chemical synthesis/*pharmacology
MH  - Animals
MH  - Aorta/drug effects
MH  - Biological Assay
MH  - Chemokine CCL2/*antagonists & inhibitors/physiology
MH  - Humans
MH  - Neovascularization, Physiologic/*drug effects
MH  - Oligopeptides/chemical synthesis/*pharmacology
MH  - Peptide Library
MH  - Rats
MH  - Receptors, CCR2
MH  - Receptors, Chemokine/drug effects/physiology
MH  - Surface Plasmon Resonance
EDAT- 2005/03/11 09:00
MHDA- 2005/04/26 09:00
CRDT- 2005/03/11 09:00
PHST- 2004/11/17 00:00 [received]
PHST- 2005/01/19 00:00 [revised]
PHST- 2005/01/21 00:00 [accepted]
PHST- 2005/03/11 09:00 [pubmed]
PHST- 2005/04/26 09:00 [medline]
PHST- 2005/03/11 09:00 [entrez]
AID - S0014-5793(05)00181-X [pii]
AID - 10.1016/j.febslet.2005.01.070 [doi]
PST - ppublish
SO  - FEBS Lett. 2005 Mar 14;579(7):1597-601. doi: 10.1016/j.febslet.2005.01.070.