PMID- 15757747
OWN - NLM
STAT- MEDLINE
DCOM- 20050801
LR  - 20061115
IS  - 0141-1136 (Print)
IS  - 0141-1136 (Linking)
VI  - 60
IP  - 2
DP  - 2005 Aug
TI  - Assessment of cytochrome P450 1A in harbour seals (Phoca vitulina) using a 
      minimally-invasive biopsy approach.
PG  - 153-69
AB  - Biomarkers of organochlorine exposure, such as the induction of cytochrome P450 
      1A (CYP1A), can be used to assess the impact of environmental contaminants on the 
      health of free-ranging marine mammal populations. The objective of the present 
      study was to measure CYP1A in skin and liver biopsies obtained from live harbour 
      seals (Phoca vitulina). Twelve harbour seal pups, aged three to five weeks, were 
      captured from the Fraser River estuary, British Columbia, Canada, and temporarily 
      held in captivity. Skin ( approximately 60 mg) and liver ( approximately 40 mg) 
      biopsies, obtained while seals were under general anaesthesia, yielded sufficient 
      tissue for the measurement of CYP1A by immunoblot analysis and ethoxyresorufin 
      O-deethylase activity. A short-term exposure experiment, in which harbour seals 
      (n=3) were treated orally with beta-naphthoflavone (BNF), resulted in increased 
      hepatic and cutaneous CYP1A protein levels, consistent with observations in other 
      mammals. This study is the first to measure CYP1A in skin and liver biopsies from 
      live harbour seals and to report in vivo BNF-associated CYP1A induction in a 
      marine mammal. The results demonstrate that microsamples collected using 
      minimally-invasive techniques can provide toxicologically-relevant information 
      form marine mammals.
FAU - Miller, Kelsey A
AU  - Miller KA
AD  - Marine Environmental Quality Section, Institute of Ocean Sciences, Fisheries and 
      Oceans Canada, P.O. Box 6000, Sidney, BC, Canada V8L 4B2.
FAU - Assuncao, Marta G L
AU  - Assuncao MG
FAU - Dangerfield, Neil J
AU  - Dangerfield NJ
FAU - Bandiera, Stelvio M
AU  - Bandiera SM
FAU - Ross, Peter S
AU  - Ross PS
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20041119
PL  - England
TA  - Mar Environ Res
JT  - Marine environmental research
JID - 9882895
RN  - 0 (Environmental Pollutants)
RN  - 6051-87-2 (beta-Naphthoflavone)
RN  - 9035-51-2 (Cytochrome P-450 Enzyme System)
RN  - EC 1.14.14.1 (Cytochrome P-450 CYP1A1)
SB  - IM
MH  - Animals
MH  - Biopsy/methods
MH  - British Columbia
MH  - Cytochrome P-450 CYP1A1/metabolism
MH  - Cytochrome P-450 Enzyme System/*metabolism
MH  - Environmental Monitoring/*methods
MH  - Environmental Pollutants/*analysis
MH  - Immunoblotting
MH  - Liver/pathology
MH  - Phoca/*metabolism
MH  - Skin/pathology
MH  - beta-Naphthoflavone
EDAT- 2005/03/11 09:00
MHDA- 2005/08/02 09:00
CRDT- 2005/03/11 09:00
PHST- 2004/01/19 00:00 [received]
PHST- 2004/10/20 00:00 [revised]
PHST- 2004/10/20 00:00 [accepted]
PHST- 2005/03/11 09:00 [pubmed]
PHST- 2005/08/02 09:00 [medline]
PHST- 2005/03/11 09:00 [entrez]
AID - S0141-1136(04)00246-6 [pii]
AID - 10.1016/j.marenvres.2004.10.002 [doi]
PST - ppublish
SO  - Mar Environ Res. 2005 Aug;60(2):153-69. doi: 10.1016/j.marenvres.2004.10.002. 
      Epub 2004 Nov 19.