PMID- 15759034
OWN - NLM
STAT- MEDLINE
DCOM- 20050610
LR  - 20130304
IS  - 0887-6924 (Print)
IS  - 0887-6924 (Linking)
VI  - 19
IP  - 5
DP  - 2005 May
TI  - Good prognosis cytogenetics in B-cell chronic lymphocytic leukemia is associated 
      in vitro with low susceptibility to apoptosis and enhanced immunogenicity.
PG  - 759-66
AB  - Chromosomal abnormalities in B-cell chronic lymphocytic leukemia (B-CLL) have 
      been shown to correlate with prognosis. Little is known about the relationship 
      between chromosomal abnormalities and biological behavior of B-CLL cells in 
      vitro. The present study was designed to explore the impact of chromosomal 
      abnormalities determined by interphase fluorescence in situ hybridization (FISH) 
      on the in vitro survival and immunogenicity of B-CLL. Considerable heterogeneity 
      was noted in the in vitro survival and expression of costimulatory, adhesion, and 
      antigen-presenting molecules by B-CLL cells. Spontaneous apoptosis of B-CLL cells 
      in vitro was significantly lower in samples with good prognosis cytogenetics when 
      compared to samples with poor prognosis cytogenetics. In contrast, B-CLL cells 
      from samples with good prognosis cytogenetics exhibited higher basal expression 
      of molecules involved in costimulation, cellular adhesion, and antigen 
      presentation, and induced significantly more T-cell proliferation in mixed 
      lymphocyte cultures. We conclude that chromosomal aberrations of B-CLL cells 
      correlate with the in vitro biological behavior of B-CLL. Our data indicate that 
      good prognosis cytogenetics correlates with less spontaneous apoptosis but 
      greater in vitro immunogenicity. These findings could have significant 
      implications on the design of future therapeutic approaches in patients with CLL, 
      and the likelihood of response based on cytogenetics.
FAU - Jahrsdorfer, B
AU  - Jahrsdorfer B
AD  - Department of Internal Medicine, The Holden Comprehensive Cancer Center, 
      University of Iowa, Iowa City, IA 52242, USA.
FAU - Wooldridge, J E
AU  - Wooldridge JE
FAU - Blackwell, S E
AU  - Blackwell SE
FAU - Taylor, C M
AU  - Taylor CM
FAU - Link, B K
AU  - Link BK
FAU - Weiner, G J
AU  - Weiner GJ
LA  - eng
GR  - P50 CA97274/CA/NCI NIH HHS/United States
GR  - R01 CA77764/CA/NCI NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Leukemia
JT  - Leukemia
JID - 8704895
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
SB  - IM
MH  - Aged
MH  - Apoptosis/*physiology
MH  - Cell Survival/physiology
MH  - Chromosome Aberrations
MH  - *Cytogenetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - L-Lactate Dehydrogenase/blood
MH  - *Leukemia, Lymphocytic, Chronic, B-Cell/genetics/immunology
MH  - Male
MH  - Middle Aged
MH  - Phenotype
MH  - Prognosis
MH  - Proto-Oncogene Proteins c-bcl-2/genetics
MH  - Tumor Cells, Cultured
EDAT- 2005/03/11 09:00
MHDA- 2005/06/11 09:00
CRDT- 2005/03/11 09:00
PHST- 2005/03/11 09:00 [pubmed]
PHST- 2005/06/11 09:00 [medline]
PHST- 2005/03/11 09:00 [entrez]
AID - 2403694 [pii]
AID - 10.1038/sj.leu.2403694 [doi]
PST - ppublish
SO  - Leukemia. 2005 May;19(5):759-66. doi: 10.1038/sj.leu.2403694.