PMID- 15760378
OWN - NLM
STAT- MEDLINE
DCOM- 20050714
LR  - 20181113
IS  - 1046-7408 (Print)
IS  - 1046-7408 (Linking)
VI  - 53
IP  - 4
DP  - 2005 Apr
TI  - Homing in on the cellular immune response to HSV-2 in humans.
PG  - 172-81
AB  - PROBLEM: Genital herpes simplex infections are generally limited to epithelia and 
      neurons. Vaccines have had activity in herpes simplex virus (HSV)-seronegative 
      women only. Understanding how HSV-specific T cells traffic to infected sites may 
      assist in vaccine design. METHOD OF STUDY: Herpes simplex virus epitopes 
      recognized by HSV-specific CD8 T cells were identified and used to make 
      fluorescent human leukocyte antigen (HLA)-peptide tetramers. Molecules related to 
      lymphocyte rolling adhesion were studied by flow cytometry and cell binding. 
      HSV-specific CD4 T cells identified ex vivo by cytokine accumulation or 
      activation marker expression, or detected in vitro by 
      5-(and-6)-carboxyfluorescein diacetate, succinimidyl ester (CFSE) dilution, were 
      similarly investigated. RESULTS: Herpes simplex virus-specific T cells are 10- to 
      100-fold more prevalent in lesional skin compared with blood and greatly enriched 
      in lesions compared with normal skin. Diverse viral antigens are recognized by 
      HSV-specific T cells. Functionally active E-selectin ligand, and cutaneous 
      lymphocyte antigen (CLA), are expressed by circulating HSV-2-specific CD8 cells. 
      CD4 cells display lower levels of CLA that are dramatically up-regulated upon 
      re-stimulation with antigen. CONCLUSIONS: Herpes simplex virus-2-specific CD8 and 
      CD4 T cells differ in constitutive expression of skin homing molecules. Vaccines 
      designed to induce proper homing are postulated to have increased efficacy.
CI  - Copyright 2005 Blackwell Munksgaard.
FAU - Koelle, David M
AU  - Koelle DM
AD  - Department of Medicine, University of Washington, Seattle, WA 98104, USA. 
      viralimm@u.washington.edu
FAU - Gonzalez, Julio C
AU  - Gonzalez JC
FAU - Johnson, Andrew S
AU  - Johnson AS
LA  - eng
GR  - P01 AI030731/AI/NIAID NIH HHS/United States
GR  - R01 AI050132/AI/NIAID NIH HHS/United States
GR  - AI30731/AI/NIAID NIH HHS/United States
GR  - AI50132/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Denmark
TA  - Am J Reprod Immunol
JT  - American journal of reproductive immunology (New York, N.Y. : 1989)
JID - 8912860
RN  - 0 (Antigens, Differentiation, T-Lymphocyte)
RN  - 0 (Antigens, Neoplasm)
RN  - 0 (CTAGE1 protein, human)
RN  - 0 (E-Selectin)
RN  - 0 (Epitopes)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Receptors, Antigen, T-Cell, alpha-beta)
SB  - IM
MH  - Antigens, Differentiation, T-Lymphocyte
MH  - Antigens, Neoplasm
MH  - CD4-Positive T-Lymphocytes/*immunology
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - E-Selectin/biosynthesis
MH  - Epitopes
MH  - Female
MH  - Herpes Genitalis/immunology/pathology/*virology
MH  - Herpesvirus 2, Human/*immunology
MH  - Humans
MH  - Immunologic Memory
MH  - Leukocyte Rolling/immunology
MH  - Membrane Glycoproteins/biosynthesis
MH  - Receptors, Antigen, T-Cell, alpha-beta/immunology
MH  - Skin/immunology/metabolism/*virology
PMC - PMC1255910
MID - NIHMS3287
EDAT- 2005/03/12 09:00
MHDA- 2005/07/15 09:00
PMCR- 2008/02/01
CRDT- 2005/03/12 09:00
PHST- 2005/03/12 09:00 [pubmed]
PHST- 2005/07/15 09:00 [medline]
PHST- 2005/03/12 09:00 [entrez]
PHST- 2008/02/01 00:00 [pmc-release]
AID - AJI262 [pii]
AID - 10.1111/j.1600-0897.2005.00262.x [doi]
PST - ppublish
SO  - Am J Reprod Immunol. 2005 Apr;53(4):172-81. doi: 
      10.1111/j.1600-0897.2005.00262.x.