PMID- 15760459
OWN - NLM
STAT- MEDLINE
DCOM- 20050901
LR  - 20181201
IS  - 1462-5814 (Print)
IS  - 1462-5814 (Linking)
VI  - 7
IP  - 4
DP  - 2005 Apr
TI  - Mycobacterium bovis bacille Calmette Guerin infection of human neutrophils 
      induces CXCL8 secretion by MyD88-dependent TLR2 and TLR4 activation.
PG  - 591-601
AB  - To investigate the potential role of neutrophils in initiation of immune 
      responses to mycobacteria, we have characterized the response of human 
      neutrophils to infection with Mycobacterium bovis bacille Calmette Guerin, the 
      BCG vaccine. BCG induced transcription and secretion of the chemokine CXCL8, by 
      signalling through Toll-like receptors TLR2 and TLR4, in conjunction with the 
      adaptor protein myeloid differentiation factor 88 (MyD88). Blocking of responses 
      with antibodies revealed a difference in the kinetics of signalling through the 
      different TLRs. Anti-TLR2 antibody blocked the early phase of CXCL8 and MyD88 
      induction. Anti-TLR4 antibody blocked the late phase of induction occurring 2 h 
      after infection. The existence of a TLR/MyD88 pathway for recognition and 
      response to mycobacterial ligands provides neutrophils with the ability to drive 
      the recruitment and activation of inflammatory cells during the early phase of 
      mycobacterial infection and immunization.
FAU - Godaly, Gabriela
AU  - Godaly G
AD  - Department of Infectious Diseases and Microbiology, Division of Investigative 
      Science, Faculty of Medicine, Imperial College London, UK. 
      Gabriela.Godaly@mig.lu.se
FAU - Young, Douglas B
AU  - Young DB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - India
TA  - Cell Microbiol
JT  - Cellular microbiology
JID - 100883691
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (Antigens, Differentiation)
RN  - 0 (BCG Vaccine)
RN  - 0 (Chemokines, CXC)
RN  - 0 (Interleukin-8)
RN  - 0 (MYD88 protein, human)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (TLR2 protein, human)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 2)
RN  - 0 (Toll-Like Receptor 4)
RN  - 0 (Toll-Like Receptors)
SB  - IM
MH  - Adaptor Proteins, Signal Transducing
MH  - Animals
MH  - Antigens, Differentiation/metabolism
MH  - BCG Vaccine
MH  - Chemokines, CXC/*metabolism
MH  - Gene Expression Regulation
MH  - Humans
MH  - Interleukin-8/metabolism
MH  - Membrane Glycoproteins/metabolism
MH  - Mycobacterium bovis/*pathogenicity
MH  - Myeloid Differentiation Factor 88
MH  - Neutrophils/immunology/*microbiology
MH  - Receptors, Cell Surface/metabolism
MH  - Receptors, Immunologic/metabolism
MH  - Signal Transduction
MH  - Toll-Like Receptor 2
MH  - Toll-Like Receptor 4
MH  - Toll-Like Receptors
EDAT- 2005/03/12 09:00
MHDA- 2005/09/02 09:00
CRDT- 2005/03/12 09:00
PHST- 2005/03/12 09:00 [pubmed]
PHST- 2005/09/02 09:00 [medline]
PHST- 2005/03/12 09:00 [entrez]
AID - CMI489 [pii]
AID - 10.1111/j.1462-5822.2004.00489.x [doi]
PST - ppublish
SO  - Cell Microbiol. 2005 Apr;7(4):591-601. doi: 10.1111/j.1462-5822.2004.00489.x.