PMID- 15763339
OWN - NLM
STAT- MEDLINE
DCOM- 20050721
LR  - 20201209
IS  - 0168-8278 (Print)
IS  - 0168-8278 (Linking)
VI  - 42
IP  - 4
DP  - 2005 Apr
TI  - Hepatitis B virus X protein promotes cell migration by inducing matrix 
      metalloproteinase-3.
PG  - 520-7
AB  - BACKGROUND/AIMS: Hepatitis B virus (HBV), a major causative agent of 
      hepatocellular carcinoma (HCC), encodes an oncogenic X protein (HBx) that 
      transcriptionally activates multiple viral and cellular promoters. How this 
      regulation influences HCC is unclear. METHODS: Global gene expression in 
      HBx-expressing and non-expressing hepatoma cells was analyzed using cDNA 
      microarrays. RESULTS: Genes that were markedly up- or down-regulated in the 
      presence of HBx included those involved in signal transduction, metabolism, 
      apoptosis, cytokine production, cell cycle, cell adhesion and oncogenesis. Other 
      genes of ill-defined function were also affected. Trans-activation proficient HBx 
      up-regulated the transcription, translation and secretion of matrix 
      metalloproteinase-3 (MMP-3), manifest as a cell migratory phenotype. This HBx 
      effect was abrogated in the presence of a MMP-3 specific peptide inhibitor. 
      CONCLUSIONS: HBx exerts selective transcriptional control in hepatoma cells and 
      induces cellular migration through the activation of MMP-3.
FAU - Yu, Feng-Ling
AU  - Yu FL
AD  - Department of Veterinary Medicine, National Pingtung University of Science and 
      Technology, Pingtung, Taiwan, ROC.
FAU - Liu, Hung-Jen
AU  - Liu HJ
FAU - Lee, Jeng-Woei
AU  - Lee JW
FAU - Liao, Ming-Huei
AU  - Liao MH
FAU - Shih, Wen-Ling
AU  - Shih WL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050108
PL  - Netherlands
TA  - J Hepatol
JT  - Journal of hepatology
JID - 8503886
RN  - 0 (Trans-Activators)
RN  - 0 (Transcription Factor AP-1)
RN  - 0 (Viral Regulatory and Accessory Proteins)
RN  - 0 (hepatitis B virus X protein)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Apoptosis/physiology/radiation effects
MH  - Carcinoma, Hepatocellular/metabolism/pathology/*virology
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Hepatitis B virus/*physiology
MH  - Hepatitis B, Chronic/metabolism/pathology/*virology
MH  - Humans
MH  - Liver Neoplasms/metabolism/pathology/*virology
MH  - Matrix Metalloproteinase 3/genetics/*metabolism
MH  - Oligonucleotide Array Sequence Analysis
MH  - Phenotype
MH  - Trans-Activators/*genetics/metabolism
MH  - Transcription Factor AP-1/metabolism
MH  - Transcription, Genetic
MH  - Ultraviolet Rays
MH  - Viral Regulatory and Accessory Proteins
EDAT- 2005/03/15 09:00
MHDA- 2005/07/22 09:00
CRDT- 2005/03/15 09:00
PHST- 2004/07/23 00:00 [received]
PHST- 2004/11/05 00:00 [revised]
PHST- 2004/11/12 00:00 [accepted]
PHST- 2005/03/15 09:00 [pubmed]
PHST- 2005/07/22 09:00 [medline]
PHST- 2005/03/15 09:00 [entrez]
AID - S0168-8278(04)00595-1 [pii]
AID - 10.1016/j.jhep.2004.11.031 [doi]
PST - ppublish
SO  - J Hepatol. 2005 Apr;42(4):520-7. doi: 10.1016/j.jhep.2004.11.031. Epub 2005 Jan 
      8.