PMID- 15764082
OWN - NLM
STAT- MEDLINE
DCOM- 20050324
LR  - 20121115
IS  - 0271-3683 (Print)
IS  - 0271-3683 (Linking)
VI  - 29
IP  - 6
DP  - 2004 Dec
TI  - Adeno-associated viruses containing bFGF or BDNF are neuroprotective against 
      excitotoxicity.
PG  - 379-86
AB  - PURPOSE: Brain-derived neurotrophic factor (BDNF) and basic fibroblast growth 
      factor (bFGF) hold much promise for the protection of retinal ganglion cells 
      against excitotoxic cell death. We tested the possibility of delivering these 
      growth factors to retinal ganglion cells via an adeno-associated viral (AAV) 
      vector and tested their efficacy in two models of excitotoxicity. METHODS: Rat 
      retinas were infected with AAV vectors encoding bFGF or BDNF. A control vector 
      containing green fluorescent protein (GFP) was injected in the contralateral eye. 
      Eyes were subjected to either an intravitreal injection of N-methyl-D-aspartate 
      (NMDA) or optic nerve crush, and ganglion cell survival was evaluated. RESULTS: 
      AAV.CMV.bFGF and AAV.CBA.BDNF were neuroprotective against NMDA injection 1 month 
      post-treatment. Additionally, AAV.CMV.bFGF was protective against optic nerve 
      crush. CONCLUSION: AAV-mediated delivery of bFGF and BDNF can promote retinal 
      cell survival following excitotoxic insult.
FAU - Schuettauf, Frank
AU  - Schuettauf F
AD  - Department of Ophthalmology, University of Pennsylvania, Scheie Eye Institute, 
      Philadelphia, Pennsylvania, USA. fschuettauf@uni-tuebingen.de
FAU - Vorwerk, Christian
AU  - Vorwerk C
FAU - Naskar, Rita
AU  - Naskar R
FAU - Orlin, Anton
AU  - Orlin A
FAU - Quinto, Kristine
AU  - Quinto K
FAU - Zurakowski, David
AU  - Zurakowski D
FAU - Dejneka, Nadine S
AU  - Dejneka NS
FAU - Klein, Ronald L
AU  - Klein RL
FAU - Meyer, Edward M
AU  - Meyer EM
FAU - Bennett, Jean
AU  - Bennett J
LA  - eng
GR  - EY 10009/EY/NEI NIH HHS/United States
GR  - R01 EY10820/EY/NEI NIH HHS/United States
GR  - R01 EY12156/EY/NEI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Curr Eye Res
JT  - Current eye research
JID - 8104312
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Excitatory Amino Acid Agonists)
RN  - 0 (Neuroprotective Agents)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 147336-22-9 (Green Fluorescent Proteins)
RN  - 6384-92-5 (N-Methylaspartate)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Brain-Derived Neurotrophic Factor/*genetics/metabolism
MH  - Cell Survival/physiology
MH  - Cytoprotection
MH  - Dependovirus/*genetics
MH  - Excitatory Amino Acid Agonists/toxicity
MH  - Fibroblast Growth Factor 2/*genetics/metabolism
MH  - Gene Expression Regulation/physiology
MH  - Genetic Therapy/methods
MH  - Genetic Vectors
MH  - Green Fluorescent Proteins/genetics
MH  - N-Methylaspartate/*toxicity
MH  - Nerve Crush
MH  - Neuroprotective Agents
MH  - Optic Nerve/pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Retinal Diseases/metabolism/pathology/*prevention & control
MH  - Retinal Ganglion Cells/drug effects/*physiology
MH  - Transfection
EDAT- 2005/03/15 09:00
MHDA- 2005/03/25 09:00
CRDT- 2005/03/15 09:00
PHST- 2005/03/15 09:00 [pubmed]
PHST- 2005/03/25 09:00 [medline]
PHST- 2005/03/15 09:00 [entrez]
AID - LHTAJ2BQK2PX8916 [pii]
AID - 10.1080/02713680490517872 [doi]
PST - ppublish
SO  - Curr Eye Res. 2004 Dec;29(6):379-86. doi: 10.1080/02713680490517872.