PMID- 15764282
OWN - NLM
STAT- MEDLINE
DCOM- 20050628
LR  - 20050314
IS  - 1052-0295 (Print)
IS  - 1052-0295 (Linking)
VI  - 79
IP  - 5-6
DP  - 2004 Oct-Dec
TI  - Simultaneous visualization of rDNA clusters and the nucleolus by combining 
      fluorescence in situ hybridization and silver staining.
PG  - 163-7
AB  - We designed two procedures to visualize simultaneously clusters of ribosomal RNA 
      genes (rDNA) and the nucleolus in plant cells. The procedures combine 
      fluorescence in situ hybridization (FISH) to visualize the rDNA clusters and 
      silver staining to observe the nucleolus. When FISH is followed by silver 
      staining, many minute FISH signals are localized in the nucleolus, and several 
      large FISH signals are seen on the nucleolar periphery. When FISH was applied to 
      the specimens with silver nitrate staining, large FISH signals were visualized in 
      the nucleoplasm associated with the nucleolar periphery, but no signals were seen 
      in the nucleoli. Thus, the two combinations of FISH and silver staining provided 
      different details regarding the arrangement of rDNA clusters in the nucleolus of 
      plant cells.
FAU - Kasai, K
AU  - Kasai K
AD  - Department of Biology and Earth Sciences, Faculty of Science, Ehime University, 
      Matsuyama, Japan.
FAU - Ishii, T
AU  - Ishii T
FAU - Sato, S
AU  - Sato S
LA  - eng
PT  - Journal Article
PL  - England
TA  - Biotech Histochem
JT  - Biotechnic & histochemistry : official publication of the Biological Stain 
      Commission
JID - 9107378
RN  - 0 (DNA, Ribosomal)
SB  - IM
MH  - Allium/genetics/ultrastructure
MH  - Cell Nucleolus/*chemistry
MH  - DNA, Ribosomal/*chemistry
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Microscopy, Fluorescence
MH  - Silver Staining/*methods
EDAT- 2005/03/15 09:00
MHDA- 2005/06/29 09:00
CRDT- 2005/03/15 09:00
PHST- 2005/03/15 09:00 [pubmed]
PHST- 2005/06/29 09:00 [medline]
PHST- 2005/03/15 09:00 [entrez]
AID - MQJYHPT3YEBT33TP [pii]
AID - 10.1080/10520290400016736 [doi]
PST - ppublish
SO  - Biotech Histochem. 2004 Oct-Dec;79(5-6):163-7. doi: 10.1080/10520290400016736.