PMID- 15764732
OWN - NLM
STAT- MEDLINE
DCOM- 20050714
LR  - 20220309
IS  - 0022-3565 (Print)
IS  - 0022-3565 (Linking)
VI  - 313
IP  - 3
DP  - 2005 Jun
TI  - Primate trace amine receptor 1 modulation by the dopamine transporter.
PG  - 983-94
AB  - Recently identified trace amine receptors are potential direct targets for drugs 
      of abuse, including amphetamine and 3,4-methylenedioxymethamphetamine (MDMA). We 
      cloned full-length rhesus monkey trace amine receptor 1 (rhTA(1)) that was 96% 
      homologous to human TA(1). The trace amines tyramine and beta-phenylethylamine 
      (PEA) and the monoamine transporter substrates (+/-)-amphetamine and (+/-)-MDMA 
      stimulated cAMP accumulation in rhTA(1)-expressing cell lines, as measured by a 
      cAMP response element-luciferase assay. Cocaine did not stimulate cAMP 
      accumulation in rhTA(1) cells, but it blocked [(3)H]PEA transport mediated by the 
      dopamine transporter. Cotransfection with the human dopamine transporter enhanced 
      PEA-, amphetamine-, and MDMA-mediated rhTA(1) receptor activation, but it 
      diminished tyramine activation of rhTA(1). Because TA(1) (EGFP-rhTA(1) chimera) 
      was largely intracellular, conceivably the dopamine transporter can facilitate 
      access of specific agonists to intracellular TA(1). rhTA(1) mRNA expression was 
      detected in rhesus monkey substantia nigra, implying that TA(1) may be 
      colocalized with the dopamine transporter in dopamine neurons. In summary, 
      primate TA(1) receptors are direct targets of trace amines, amphetamine, and 
      MDMA. These receptors could also be indirect targets of amphetamine, MDMA, and 
      cocaine through modification of monoamine transporter function. Conceivably, 
      rhTA(1) receptors may be located on pre- or postsynaptic membranes. Interference 
      with the carrier function of monoamine transporters with a consequent rise of 
      extracellular levels of trace amines could activate these receptors. The cloning 
      of a highly homologous TA(1) from rhesus monkey and demonstration that rhTA(1) 
      receptors are activated by drugs of abuse, indicate that nonhuman primates may 
      serve to model physiological and pharmacological TA(1)-mediated responses in 
      humans.
FAU - Miller, Gregory M
AU  - Miller GM
AD  - Division of Neurochemistry, New England Primate Research Center, Harvard Medical 
      School, Southborough, MA 01772, USA.
FAU - Verrico, Christopher D
AU  - Verrico CD
FAU - Jassen, Amy
AU  - Jassen A
FAU - Konar, Martha
AU  - Konar M
FAU - Yang, Hong
AU  - Yang H
FAU - Panas, Helen
AU  - Panas H
FAU - Bahn, Mary
AU  - Bahn M
FAU - Johnson, Ryan
AU  - Johnson R
FAU - Madras, Bertha K
AU  - Madras BK
LA  - eng
GR  - DA06303/DA/NIDA NIH HHS/United States
GR  - RR00168/RR/NCRR NIH HHS/United States
GR  - P51 OD011103/OD/NIH HHS/United States
GR  - R01 DA006303/DA/NIDA NIH HHS/United States
GR  - DA15305/DA/NIDA NIH HHS/United States
GR  - K05 DA015305/DA/NIDA NIH HHS/United States
GR  - R01 DA011558/DA/NIDA NIH HHS/United States
GR  - P51 RR000168/RR/NCRR NIH HHS/United States
GR  - DA016606/DA/NIDA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, P.H.S.
DEP - 20050311
PL  - United States
TA  - J Pharmacol Exp Ther
JT  - The Journal of pharmacology and experimental therapeutics
JID - 0376362
RN  - 0 (Dopamine Plasma Membrane Transport Proteins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Phenethylamines)
RN  - 0 (Receptors, G-Protein-Coupled)
RN  - 327C7L2BXQ (phenethylamine)
RN  - E0399OZS9N (Cyclic AMP)
RN  - I5Y540LHVR (Cocaine)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
RN  - VTD58H1Z2X (Dopamine)
RN  - XMC8VP6RI2 (Trace amine-associated receptor 1)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Cells, Cultured
MH  - Cocaine/pharmacology
MH  - Cyclic AMP/biosynthesis
MH  - Dopamine/metabolism
MH  - Dopamine Plasma Membrane Transport Proteins
MH  - Humans
MH  - Macaca mulatta
MH  - Membrane Glycoproteins/*physiology
MH  - Membrane Transport Proteins/*physiology
MH  - Molecular Sequence Data
MH  - N-Methyl-3,4-methylenedioxyamphetamine/pharmacology
MH  - Nerve Tissue Proteins/*physiology
MH  - Phenethylamines/metabolism
MH  - Rats
MH  - Receptors, G-Protein-Coupled/*drug effects/physiology
MH  - Response Elements/physiology
MH  - Substantia Nigra/chemistry
EDAT- 2005/03/15 09:00
MHDA- 2005/07/15 09:00
CRDT- 2005/03/15 09:00
PHST- 2005/03/15 09:00 [pubmed]
PHST- 2005/07/15 09:00 [medline]
PHST- 2005/03/15 09:00 [entrez]
AID - jpet.105.084459 [pii]
AID - 10.1124/jpet.105.084459 [doi]
PST - ppublish
SO  - J Pharmacol Exp Ther. 2005 Jun;313(3):983-94. doi: 10.1124/jpet.105.084459. Epub 
      2005 Mar 11.