PMID- 15765056 OWN - NLM STAT- MEDLINE DCOM- 20050418 LR - 20131121 IS - 1225-8873 (Print) IS - 1225-8873 (Linking) VI - 43 IP - 1 DP - 2005 Feb TI - Optimization of lactic acid production in SSF by Lactobacillus amylovorus NRRL B-4542 using Taguchi methodology. PG - 38-43 AB - Lactic acid production parameter optimization using Lactobacillus amylovorus NRRL B-4542 was performed using the design of experiments (DOE) available in the form of an orthogonal array and a software for automatic design and analysis of the experiments, both based on Taguchi protocol. Optimal levels of physical parameters and key media components namely temperature, pH, inoculum size, moisture, yeast extract, MgSO4 . 7H20, Tween 80, and corn steep liquor (CSL) were determined. Among the physical parameters, temperature contributed higher influence, and among media components, yeast extract, MgSO4 . 7H20, and Tween 80 played important roles in the conversion of starch to lactic acid. The expected yield of lactic acid under these optimal conditions was 95.80% and the actual yield at optimum conditions was 93.50%. FAU - Nagarjun, Pyde Acharya AU - Nagarjun PA AD - Department of Microbiology, Osmania University, Hyderabad-500 007, India. FAU - Rao, Ravella Sreenivas AU - Rao RS FAU - Rajesham, Swargam AU - Rajesham S FAU - Rao, Linga Venkateswar AU - Rao LV LA - eng PT - Journal Article PL - Korea (South) TA - J Microbiol JT - Journal of microbiology (Seoul, Korea) JID - 9703165 RN - 0 (Culture Media) RN - 33X04XA5AT (Lactic Acid) RN - 9005-25-8 (Starch) SB - IM MH - Bacteriological Techniques MH - Culture Media MH - Fermentation MH - Hydrogen-Ion Concentration MH - Lactic Acid/*biosynthesis MH - Lactobacillus acidophilus/*metabolism MH - Starch/metabolism MH - Temperature EDAT- 2005/03/15 09:00 MHDA- 2005/04/19 09:00 CRDT- 2005/03/15 09:00 PHST- 2005/03/15 09:00 [pubmed] PHST- 2005/04/19 09:00 [medline] PHST- 2005/03/15 09:00 [entrez] AID - 2140 [pii] PST - ppublish SO - J Microbiol. 2005 Feb;43(1):38-43.