PMID- 15765474
OWN - NLM
STAT- MEDLINE
DCOM- 20050929
LR  - 20061115
IS  - 0173-0835 (Print)
IS  - 0173-0835 (Linking)
VI  - 26
IP  - 7-8
DP  - 2005 Apr
TI  - On-line concentration of proteins in pressurized capillary electrochromatography 
      coupled with electrospray ionization-mass spectrometry.
PG  - 1398-405
AB  - Pressurized capillary electrochromatography (pCEC) and electrospray 
      ionization-mass spectrometry (ESI-MS) have been hyphenated for protein analysis. 
      Taken cytochrome c, lysozyme, and insulin as samples, the limits of detection 
      (LODs) for absolute concentrations are 10(-11) mol (signal-to-noise ratio S/N = 
      3) with relative standard deviations (RSDs) of retention time and peak area, 
      respectively, of less than 1.7% and 4.8%. In order to improve the detection 
      sensitivity, on-line concentration by field-enhanced sample-stacking effect and 
      chromatographic zone-sharpening effect has been developed, and parameters 
      affecting separation and detection, such as pH and electrolyte concentration in 
      the mobile phase, separation voltage, as well as enrichment voltage and time, 
      have been studied systematically. Under the optimized conditions, the LODs of the 
      three proteins could be decreased up to 100-fold. In addition, the feasibility of 
      such techniques has been further demonstrated by the analysis of modified 
      insulins at a concentration of 20 microg/mL.
FAU - Liang, Zhen
AU  - Liang Z
AD  - National Chromatography Research & Analysis Center, Dalian Institute of Chemical 
      Physics, Chinese Academy of Sciences, Dalian, China.
FAU - Zhang, Lihua
AU  - Zhang L
FAU - Duan, Jicheng
AU  - Duan J
FAU - Yan, Chao
AU  - Yan C
FAU - Zhang, Weibing
AU  - Zhang W
FAU - Zhang, Yukui
AU  - Zhang Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Electrophoresis
JT  - Electrophoresis
JID - 8204476
RN  - 0 (Proteins)
SB  - IM
MH  - Chromatography, Micellar Electrokinetic Capillary/*methods
MH  - Pressure
MH  - Proteins/*chemistry
MH  - Spectrometry, Mass, Electrospray Ionization/*methods
EDAT- 2005/03/15 09:00
MHDA- 2005/09/30 09:00
CRDT- 2005/03/15 09:00
PHST- 2005/03/15 09:00 [pubmed]
PHST- 2005/09/30 09:00 [medline]
PHST- 2005/03/15 09:00 [entrez]
AID - 10.1002/elps.200406200 [doi]
PST - ppublish
SO  - Electrophoresis. 2005 Apr;26(7-8):1398-405. doi: 10.1002/elps.200406200.