PMID- 15768984
OWN - NLM
STAT- MEDLINE
DCOM- 20100603
LR  - 20151119
IS  - 1000-0593 (Print)
IS  - 1000-0593 (Linking)
VI  - 24
IP  - 1
DP  - 2004 Jan
TI  - [Studies on application and mechanism of energy transfer system of acridine 
      orange (AO)-rhodamine B (RB) dimer in the determination of DNA].
PG  - 85-8
AB  - Not only dye monomer is the form of energy transfer but also dye dimer is the 
      effective form of energy transfer. In this work, the energy transfer system of 
      acridine orange (AO)-rhodamine B (RB) dimer was built for the determination of 
      DNA as fluorescence probe. At the same time, the mechanism was also considered. 
      Under the optimum experimental conditions, the linear range of this assay was 
      0.33-1.33 mg x L(-1) for fish sperm DNA and 0.33-3.33 mg x L(-1) for calf thymus 
      DNA, the detection limit was 1.63 x 10(-3) mg x L(-1) for fish sperm DNA and 1.52 
      x 10(-3) mg x L(-1) for calf thymus DNA, respectively. The RSD for the 
      determination of 1.00 mg x L(-1) DNA was 2.40% for fsDNA and 2.00% for ctDNA, 
      respectively. The method was used for the determination of DNA in synthetic 
      samples with satisfied results.
FAU - Gao, Feng
AU  - Gao F
AD  - College of Chemistry and Material Science, Anhui Normal University, Wuhu 241000, 
      China.
FAU - Zhu, Chang-qing
AU  - Zhu CQ
FAU - Wang, Lun
AU  - Wang L
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Guang Pu Xue Yu Guang Pu Fen Xi
JT  - Guang pu xue yu guang pu fen xi = Guang pu
JID - 9424805
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Rhodamines)
RN  - 9007-49-2 (DNA)
RN  - 91080-16-9 (calf thymus DNA)
RN  - F30N4O6XVV (Acridine Orange)
RN  - K7G5SCF8IL (rhodamine B)
SB  - IM
MH  - Acridine Orange/*chemistry
MH  - DNA/*chemistry
MH  - *Fluorescent Dyes
MH  - Limit of Detection
MH  - Rhodamines/*chemistry
MH  - Spectrometry, Fluorescence/*methods
MH  - Static Electricity
EDAT- 2005/03/17 09:00
MHDA- 2010/06/04 06:00
CRDT- 2005/03/17 09:00
PHST- 2005/03/17 09:00 [pubmed]
PHST- 2010/06/04 06:00 [medline]
PHST- 2005/03/17 09:00 [entrez]
PST - ppublish
SO  - Guang Pu Xue Yu Guang Pu Fen Xi. 2004 Jan;24(1):85-8.