PMID- 15769125
OWN - NLM
STAT- MEDLINE
DCOM- 20050428
LR  - 20131121
IS  - 0021-8561 (Print)
IS  - 0021-8561 (Linking)
VI  - 53
IP  - 6
DP  - 2005 Mar 23
TI  - Antiproliferative effects of dietary phenolic substances and hydrogen peroxide.
PG  - 1990-5
AB  - There has been controversy as to whether the antiproliferative activity of 
      dietary phenolic substances on cancer cells is due to the bioactivities of 
      phenolics or the generation of hydrogen peroxide (H2O2) in media as an artifact. 
      This study was to investigate whether the formation of H2O2 by different 
      phenolics induces acute toxicity and carcinogenicity in normal rat liver 
      epithelial cells. Gallic acid, one of the major antioxidants present in fruits 
      and vegetables, dose-dependently generated considerably more H2O2 in DMEM media 
      without cells than did quercetin. Gallic acid exerted stronger antiproliferative 
      activity than quercetin on both Caco-2 human colon cancer cells (Caco-2 cells) 
      and WB-F344 normal rat liver epithelial cells (WB cells) cultured in DMEM media, 
      and the effect was partially reduced by catalase. Furthermore, gallic acid (but 
      not quercetin) also inhibited gap-junction intercellular communication (GJIC; a 
      carcinogenic phenomenon), which was in part protected by the addition of 
      catalase. Exogenous H2O2 addition also inhibited the proliferation of both Caco-2 
      cells and WB cells and inhibited GJIC in a dose-dependent manner, but these 
      effects were almost abolished by the treatment with catalase. From these results 
      it is concluded that the antiproliferative effects of some antioxidants on cancer 
      cells are partially due to their prooxidant actions.
FAU - Lee, Ki Won
AU  - Lee KW
AD  - Department of Food Science and Technology, Center for Agricultural Biomaterials, 
      School of Agricultural Biotechnology, Seoul National University, Seoul 152-741, 
      Republic of Korea.
FAU - Hur, Haeng Jeon
AU  - Hur HJ
FAU - Lee, Hyong Joo
AU  - Lee HJ
FAU - Lee, Chang Yong
AU  - Lee CY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Antioxidants)
RN  - 0 (Phenols)
RN  - 632XD903SP (Gallic Acid)
RN  - 9IKM0I5T1E (Quercetin)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.11.1.6 (Catalase)
SB  - IM
MH  - Animals
MH  - Antioxidants/*pharmacology
MH  - Caco-2 Cells
MH  - Catalase/pharmacology
MH  - Cell Division/*drug effects
MH  - Cell Line
MH  - *Diet
MH  - Gallic Acid/pharmacology
MH  - Gap Junctions/drug effects
MH  - Humans
MH  - Hydrogen Peroxide/*metabolism
MH  - Liver
MH  - Phenols/*pharmacology
MH  - Quercetin/pharmacology
MH  - Rats
EDAT- 2005/03/17 09:00
MHDA- 2005/04/29 09:00
CRDT- 2005/03/17 09:00
PHST- 2005/03/17 09:00 [pubmed]
PHST- 2005/04/29 09:00 [medline]
PHST- 2005/03/17 09:00 [entrez]
AID - 10.1021/jf0486040 [doi]
PST - ppublish
SO  - J Agric Food Chem. 2005 Mar 23;53(6):1990-5. doi: 10.1021/jf0486040.