PMID- 15769483
OWN - NLM
STAT- MEDLINE
DCOM- 20050505
LR  - 20131121
IS  - 0024-3205 (Print)
IS  - 0024-3205 (Linking)
VI  - 76
IP  - 22
DP  - 2005 Apr 15
TI  - Long-term administration of aqueous garlic extract (AGE) alleviates liver 
      fibrosis and oxidative damage induced by biliary obstruction in rats.
PG  - 2593-606
AB  - The aim of this study was to assess the antioxidant and antifibrotic effects of 
      chronic administration of aqueous garlic extract on liver fibrosis induced by 
      biliary obstruction in rats. Liver fibrosis was induced in male Wistar albino 
      rats by bile duct ligation and scission (BDL). Aqueous garlic extract (AGE, 1 
      ml/kg, i.p., corresponding to 250 mg/kg) or saline was administered for 28 days. 
      At the end of the experiment, rats were killed by decapitation. Serum aspartate 
      aminotransferase (AST), alanine aminotransferase (ALT) and lactate dehydrogenase 
      (LDH) levels were determined to assess liver functions and tissue damage, 
      respectively. Tumor necrosis factor-alpha (TNF-alpha) was also assayed in serum 
      samples. Liver tissues were taken for determination of the free radicals, renal 
      malondialdehyde (MDA) levels, an end product of lipid peroxidation; glutathione 
      (GSH) levels, a key antioxidant; and myeloperoxidase (MPO) activity, as an 
      indirect index of neutrophil infiltration. Hepatic collagen content, as a 
      fibrosis marker was also determined. Serum AST, ALT, LDH, and TNF- alpha levels 
      were elevated in the BDL group as compared to control group, while this increase 
      was significantly decreased by AGE treatment. Hepatic GSH levels, significantly 
      depressed by BDL, were elevated back to control levels in AGE-treated BDL group. 
      Increases in tissue free radical and MDA levels and MPO activity due to BDL were 
      reduced back to control levels by AGE treatment. Similarly, increased hepatic 
      collagen content in the BDL rats was reduced to the level of the control group 
      with AGE treatment. Since AGE administration alleviated the BDL-induced oxidative 
      injury of the liver and improved the hepatic structure and function, it seems 
      likely that AGE with its antioxidant and antifibrotic properties, may be of 
      potential therapeutic value in protecting the liver fibrosis and oxidative injury 
      due to biliary obstruction.
FAU - Gedik, Nursal
AU  - Gedik N
AD  - Kasimpasa Military Hospital, Division of Biochemistry, Istanbul, Turkey.
FAU - Kabasakal, Levent
AU  - Kabasakal L
FAU - Sehirli, Ozer
AU  - Sehirli O
FAU - Ercan, Feriha
AU  - Ercan F
FAU - Sirvanci, Serap
AU  - Sirvanci S
FAU - Keyer-Uysal, Meral
AU  - Keyer-Uysal M
FAU - Sener, Goksel
AU  - Sener G
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Plant Extracts)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - 9007-34-5 (Collagen)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 1.11.1.7 (Peroxidase)
RN  - EC 2.6.1.2 (Alanine Transaminase)
RN  - GAN16C9B8O (Glutathione)
SB  - IM
MH  - Alanine Transaminase/analysis
MH  - Animals
MH  - Bile Ducts/surgery
MH  - Collagen/analysis
MH  - Garlic/*chemistry
MH  - Glutathione/analysis
MH  - L-Lactate Dehydrogenase/analysis
MH  - Ligation
MH  - Lipid Peroxidation/drug effects
MH  - Liver/pathology/physiopathology
MH  - Liver Cirrhosis, Experimental/*drug therapy/pathology/physiopathology
MH  - Male
MH  - Malondialdehyde/analysis
MH  - Oxidation-Reduction/drug effects
MH  - Peroxidase/analysis
MH  - Plant Extracts/*administration & dosage/chemistry
MH  - Rats
MH  - Rats, Wistar
MH  - Tumor Necrosis Factor-alpha/analysis
EDAT- 2005/03/17 09:00
MHDA- 2005/05/06 09:00
CRDT- 2005/03/17 09:00
PHST- 2004/09/15 00:00 [received]
PHST- 2004/11/16 00:00 [accepted]
PHST- 2005/03/17 09:00 [pubmed]
PHST- 2005/05/06 09:00 [medline]
PHST- 2005/03/17 09:00 [entrez]
AID - S0024-3205(05)00050-0 [pii]
AID - 10.1016/j.lfs.2004.11.021 [doi]
PST - ppublish
SO  - Life Sci. 2005 Apr 15;76(22):2593-606. doi: 10.1016/j.lfs.2004.11.021.