PMID- 15771142
OWN - NLM
STAT- MEDLINE
DCOM- 20050519
LR  - 20220331
IS  - 1426-9686 (Print)
IS  - 1426-9686 (Linking)
VI  - 17
IP  - 102
DP  - 2004 Dec
TI  - [Significance of lactate dehydrogenase measurements in diagnosis of 
      malignancies].
PG  - 644-7
AB  - The clinical stage of cancer is the end result in a complex, multistage 
      carcinogenic process, during which several regulatory circuits are altered. These 
      alterations often manifest themselves through changes in the cellular enzymatic 
      patterns. It is well known that the most prominent biochemical feature of 
      malignant cells remains the high glycolytic activity, the result of which is 
      production of a large quantity of lactic acid. Lactic acid dehydrogenase (LDH) is 
      considered a key enzyme in glycolysis. It catalyses the process of lactate 
      production in pirogronian reduction reaction. Five active isoenzymes of LDH can 
      be found in human tissues. Each isoenzyme is a tetrametric composition of two 
      subunits, A(M) and B(H), encoded by separate genes (Ldh-A and Ldh-B 
      respectively). Each subunit determines metabolic characteristics of isoenzymes 
      and predisposes them to activity either in aerobic (B-type isoenzymes) or 
      anaerobic environment (A-type isoenzymes). Therefore each normal tissue has its 
      own special LDH activity pattern, depending on its function. In malignant tumours 
      the macromolecular synthesis is changed in favour of subunit A in comparison with 
      the healthy tissues from which they derive. It results in a dominance of the 
      cathodic isoenzymes, which move slowest after electrophoresis, namely LD5 and 
      LD4. It is even thought that izoenzymograms of most malignant tumours do not 
      differ. Similar changes in isoenzyme activity of LDH are observed in 
      morphologically normal tissues, adjacent to the tumour, which can suggest early 
      pre-tumour stage in those tissues. In this report we summarise basic 
      physico-chemical properties of LDH and discuss its clinical significance in 
      diagnosis and prognosis of neoplastic diseases.
FAU - Augoff, Katarzyna
AU  - Augoff K
AD  - Klinika Chirurgii Przewodu Pokarmowego i Chirurgii Ogolnej Akademii Medycznej we 
      Wroclawiu. augofka@poczta.onet.pl
FAU - Grabowski, Krzysztof
AU  - Grabowski K
LA  - pol
PT  - English Abstract
PT  - Journal Article
PT  - Review
TT  - Przydatnosc oznaczania dehydrogenazy mleczanowej w rozpoznawaniu chorob 
      nowotworowych.
PL  - Poland
TA  - Pol Merkur Lekarski
JT  - Polski merkuriusz lekarski : organ Polskiego Towarzystwa Lekarskiego
JID - 9705469
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
SB  - IM
MH  - Humans
MH  - L-Lactate Dehydrogenase/*metabolism
MH  - Neoplasms/*diagnosis/*enzymology
RF  - 31
EDAT- 2005/03/18 09:00
MHDA- 2005/05/20 09:00
CRDT- 2005/03/18 09:00
PHST- 2005/03/18 09:00 [pubmed]
PHST- 2005/05/20 09:00 [medline]
PHST- 2005/03/18 09:00 [entrez]
PST - ppublish
SO  - Pol Merkur Lekarski. 2004 Dec;17(102):644-7.