PMID- 15771903
OWN - NLM
STAT- MEDLINE
DCOM- 20050421
LR  - 20050317
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 158
IP  - 1
DP  - 2005 Apr 1
TI  - Identification of cell lineages involved by t(15;17) in acute promyelocytic 
      leukemia by combined fluorescence activated cell sorting and FISH.
PG  - 43-8
AB  - Bone marrow cells from five patients with acute promyelocytic leukemia (APL) with 
      t(15;17) were studied by a combination of fluorescence activated cell sorting and 
      fluorescence in situ hybridization (FISH) to establish the cell lineage 
      involvement of t(15;17). Interphase FISH demonstrated that the fusion gene 
      (PML/RARA) was present in almost all abnormal promyelocytes. In one case, the 
      translocation was demonstrated in both CD34+ and CD34- APL cells. The t(15;17) 
      abnormality was not detectable in erythroblasts nor in T- or B-lymphoid cells. 
      These results suggest that lymphocytes and erythroblasts are not clonally 
      involved in APL, and that malignant transformation in some cases of APL may occur 
      at the level of CD34+ cells.
FAU - Chang, Hong
AU  - Chang H
AD  - Department of Laboratory Hematology, Princess Margaret Hospital/University Health 
      Network, University of Toronto, 610 University Avenue, 4-320, Toronto, Ontario 
      M5G-2M9, Canada. hong.chang@uhn.on.ca
FAU - Qi, Xiao Ying
AU  - Qi XY
FAU - Sutherland, D Robert
AU  - Sutherland DR
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Adult
MH  - Cell Lineage
MH  - Cell Separation
MH  - *Chromosomes, Human, Pair 15
MH  - *Chromosomes, Human, Pair 17
MH  - Flow Cytometry
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Leukemia, Promyelocytic, Acute/*genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - *Translocation, Genetic
EDAT- 2005/03/18 09:00
MHDA- 2005/04/22 09:00
CRDT- 2005/03/18 09:00
PHST- 2004/07/07 00:00 [received]
PHST- 2004/08/05 00:00 [revised]
PHST- 2004/08/06 00:00 [accepted]
PHST- 2005/03/18 09:00 [pubmed]
PHST- 2005/04/22 09:00 [medline]
PHST- 2005/03/18 09:00 [entrez]
AID - S0165-4608(04)00336-X [pii]
AID - 10.1016/j.cancergencyto.2004.08.018 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2005 Apr 1;158(1):43-8. doi: 
      10.1016/j.cancergencyto.2004.08.018.