PMID- 1577279
OWN - NLM
STAT- MEDLINE
DCOM- 19920611
LR  - 20190516
IS  - 0890-9369 (Print)
IS  - 0890-9369 (Linking)
VI  - 6
IP  - 5
DP  - 1992 May
TI  - Immortalization of conditionally transformed chicken cells: loss of normal p53 
      expression is an early step that is independent of cell transformation.
PG  - 876-87
AB  - Clones of mortal chicken fibroblasts and erythroblasts transformed by 
      temperature-sensitive v-src and v-erb B oncoproteins have been developed into 
      immortal cell lines that retain the conditional transformed phenotype. The 
      expressions of two tumor suppressor genes, the retinoblastoma (Rb) gene and the 
      p53 gene, were investigated during senescence, crisis, and cell line 
      establishment. In temperature-sensitive (ts)-v-erb B erythroblasts and ts-v-src 
      fibroblasts (as well as in v-myc macrophages), loss of p53 mRNA or expression of 
      a mutated p53 gene invariably occurred in the early phase of immortalization. In 
      contrast, expression of the Rb gene was unchanged at all stages of 
      immortalization. Inactivation of the original temperature-sensitive oncogene led 
      to loss of the transformed phenotype in fibroblasts and to differentiation in 
      erythroblasts, even in lines that were immortal and lacked p53. The results 
      demonstrate that the process of immortalization is distinct from cell 
      transformation, probably requiring different mutational events.
FAU - Ulrich, E
AU  - Ulrich E
AD  - Research Institute for Molecular Pathology, Vienna, Austria.
FAU - Boehmelt, G
AU  - Boehmelt G
FAU - Bird, A
AU  - Bird A
FAU - Beug, H
AU  - Beug H
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Genes Dev
JT  - Genes & development
JID - 8711660
SB  - IM
MH  - Animals
MH  - Base Sequence
MH  - Blotting, Northern
MH  - Cell Line
MH  - Cell Transformation, Viral/*genetics
MH  - Chickens
MH  - Gene Expression/genetics
MH  - Genes, Retinoblastoma/*genetics
MH  - Genes, p53/*genetics
MH  - Molecular Sequence Data
MH  - Mutation/genetics
MH  - Oncogenes/genetics
MH  - Polymerase Chain Reaction
MH  - Temperature
EDAT- 1992/05/01 00:00
MHDA- 1992/05/01 00:01
CRDT- 1992/05/01 00:00
PHST- 1992/05/01 00:00 [pubmed]
PHST- 1992/05/01 00:01 [medline]
PHST- 1992/05/01 00:00 [entrez]
AID - 10.1101/gad.6.5.876 [doi]
PST - ppublish
SO  - Genes Dev. 1992 May;6(5):876-87. doi: 10.1101/gad.6.5.876.