PMID- 15773965
OWN - NLM
STAT- MEDLINE
DCOM- 20050620
LR  - 20220410
IS  - 0934-0874 (Print)
IS  - 0934-0874 (Linking)
VI  - 18
IP  - 4
DP  - 2005 Apr
TI  - Hypoxia-reoxygenation-induced chemokine transcription is not prevented by 
      preconditioning or intermittent hypoxia, in mice hepatocytes.
PG  - 444-52
AB  - Prolonged ischemia used in liver surgery and/or transplantation causes cellular 
      damage resulting in apoptosis and necrosis. Ischemia-reperfusion (I/R) led 
      Kupffer cells to pro-inflammatory cytokines secretion [tumor necrosis factor 
      (TNF)-alpha, interleukin-1] which involve chemokines secretion by hepatocytes. 
      These chemokines have neutrophil chemotactic properties and neutrophils are 
      involved in the development of I/R-induced necrosis. The aim of this study was to 
      specify the consequence of partial oxygen pressure variation on hepatocyte 
      chemokines synthesis and to verify if intermittent hypoxia and/or preconditioning 
      could decrease it. It was performed on primary cultured mice hepatocytes and 
      Kupffer cells, subjected to continuous, intermittent hypoxia or preconditioning 
      phases, mimicking surgical processes. The chemokine secretion was evaluated by 
      RNase protection assay and enzyme-linked immunosorbent assay method. Only 
      monocyte chemoattractant protein-1 (MCP-1) and macrophage inflammatory protein-2 
      (MIP-2) mRNA formation were observed, especially after 1-h hypoxia followed by 
      10-h (for MCP-1) or 24-h reoxygenation (for MIP-2). In conclusion, TNF-alpha and 
      coculture with Kupffer cells increased hepatocyte chemokines mRNA transcription, 
      whereas surgical split up protocols (intermittent hypoxia and preconditioning) 
      had no significant effect.
FAU - Laurens, Marina
AU  - Laurens M
AD  - Laboratoire de Recherches Chirurgicales, Faculte de Medecine, Universite de 
      Nice-Sophia Antipolis, France.
FAU - Defamie, Virginie
AU  - Defamie V
FAU - Scozzari, Gitana
AU  - Scozzari G
FAU - Schmid-Alliana, Annie
AU  - Schmid-Alliana A
FAU - Gugenheim, Jean
AU  - Gugenheim J
FAU - Crenesse, Dominique
AU  - Crenesse D
LA  - eng
PT  - Journal Article
PL  - Switzerland
TA  - Transpl Int
JT  - Transplant international : official journal of the European Society for Organ 
      Transplantation
JID - 8908516
RN  - 0 (CCL2 protein, human)
RN  - 0 (CCL8 protein, human)
RN  - 0 (Ccl8 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokine CCL8)
RN  - 0 (Chemokines)
RN  - 0 (Monocyte Chemoattractant Proteins)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - Cell Hypoxia/*physiology
MH  - Cells, Cultured
MH  - Chemokine CCL2/genetics/metabolism
MH  - Chemokine CCL8
MH  - Chemokines/*genetics
MH  - Hepatocytes/drug effects/*physiology
MH  - *Ischemic Preconditioning
MH  - Male
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Monocyte Chemoattractant Proteins/genetics/metabolism
MH  - Oxygen/*pharmacology
MH  - Transcription, Genetic/*drug effects
EDAT- 2005/03/19 09:00
MHDA- 2005/06/21 09:00
CRDT- 2005/03/19 09:00
PHST- 2005/03/19 09:00 [pubmed]
PHST- 2005/06/21 09:00 [medline]
PHST- 2005/03/19 09:00 [entrez]
AID - TRI064 [pii]
AID - 10.1111/j.1432-2277.2004.00064.x [doi]
PST - ppublish
SO  - Transpl Int. 2005 Apr;18(4):444-52. doi: 10.1111/j.1432-2277.2004.00064.x.