PMID- 15777927
OWN - NLM
STAT- MEDLINE
DCOM- 20050614
LR  - 20191210
IS  - 0022-1759 (Print)
IS  - 0022-1759 (Linking)
VI  - 297
IP  - 1-2
DP  - 2005 Feb
TI  - Detection of immune-complexed 9-O-acetylated sialoglycoconjugates in the sera of 
      patients with pediatric acute lymphoblastic leukemia.
PG  - 13-26
AB  - Although childhood acute lymphoblastic leukemia (ALL) is highly responsive to 
      chemotherapy, reliable techniques are needed to determine treatment outcome. Over 
      expression of 9-O-acetylated sialoglycoconjugates (9-OAcSGs) on lymphoblasts and 
      concomitant anti-9-OAcSGs was found to have a diagnostic and prognostic 
      potential. However, the presence of circulatory immune-complexed antigens remains 
      unknown. The present study was aimed to evaluate whether immune-complexed 
      9-OAcSGs can be harnessed for better disease management. Immune-complexed 
      antigens were evaluated in ALL sera (n=262) by a Dot-blot using a 
      9-OAcSAalpha2-6GalNAc-specific lectin, Achatinin-H. Using three serum samples, 
      the inter- and intra-assay imprecision was evaluated as 11-13% and 7-11%, 
      respectively. The recovery of spiked 9-OAcSGs was 84.2-95.4%. The central 95% 
      reference interval for immune-complexed 9-OAcSGs in normal human sera (NHS, 
      n=144) was 2.9-3.4 mug/ml irrespective of sex and age. At disease presentation, 
      the immune-complexed 9-OAcSGs were fivefold higher than NHS, decreased with 
      remission induction and importantly, reappeared with clinical relapse. Sera from 
      patients with other hematological disorders (n=86) showed negligible levels. The 
      Dot-blot demonstrated the potential application of immune-complexed antigen as a 
      disease-specific marker and its efficacy as a sensitive and specific method that 
      could serve as an economical yet effective index for monitoring disease status.
FAU - Bandyopadhyay, Suman
AU  - Bandyopadhyay S
AD  - Immunobiology Division, Indian Institute of Chemical Biology, 4 Raja S.C. Mullick 
      Road, Jadavpur, Kolkata-700 032, India.
FAU - Mukherjee, Kankana
AU  - Mukherjee K
FAU - Chatterjee, Mitali
AU  - Chatterjee M
FAU - Bhattacharya, Dilip Kumar
AU  - Bhattacharya DK
FAU - Mandal, Chitra
AU  - Mandal C
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Validation Study
DEP - 20041228
PL  - Netherlands
TA  - J Immunol Methods
JT  - Journal of immunological methods
JID - 1305440
RN  - 0 (Antigens, Tumor-Associated, Carbohydrate)
RN  - 0 (Glycoconjugates)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Lectins)
RN  - 0 (Sialic Acids)
RN  - 0 (achatinin(H))
SB  - IM
MH  - Adolescent
MH  - Antigens, Tumor-Associated, Carbohydrate/*blood
MH  - Child
MH  - Child, Preschool
MH  - Female
MH  - Glycoconjugates/*blood
MH  - Histocompatibility Antigens Class II/blood
MH  - Humans
MH  - Immunoblotting/*methods
MH  - Lectins/blood/immunology
MH  - Male
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*diagnosis
MH  - Prognosis
MH  - Sialic Acids/*blood
MH  - Treatment Outcome
EDAT- 2005/03/22 09:00
MHDA- 2005/06/15 09:00
CRDT- 2005/03/22 09:00
PHST- 2004/05/24 00:00 [received]
PHST- 2004/09/06 00:00 [revised]
PHST- 2004/11/04 00:00 [accepted]
PHST- 2005/03/22 09:00 [pubmed]
PHST- 2005/06/15 09:00 [medline]
PHST- 2005/03/22 09:00 [entrez]
AID - S0022-1759(04)00415-6 [pii]
AID - 10.1016/j.jim.2004.11.014 [doi]
PST - ppublish
SO  - J Immunol Methods. 2005 Feb;297(1-2):13-26. doi: 10.1016/j.jim.2004.11.014. Epub 
      2004 Dec 28.