PMID- 15778382
OWN - NLM
STAT- MEDLINE
DCOM- 20050607
LR  - 20190516
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 174
IP  - 7
DP  - 2005 Apr 1
TI  - Inverse correlation of maturity and antibacterial activity in human dendritic 
      cells.
PG  - 4203-9
AB  - Dendritic cells (DCs) are a key part of host defense against microbial pathogens, 
      being part of the innate immune system, but also instructing the adaptive T cell 
      response. This study was designed to evaluate whether human DCs directly 
      contribute to innate immunity by killing intracellular bacteria, using 
      tuberculosis as a model. DCs were detected in bronchoalveolar lavage samples 
      indicating that DCs are available for immediate interaction with Mycobacterium 
      tuberculosis (M. Tb) after inhalation of the pathogen. The phenotype of DC in 
      bronchoalveolar lavage closely resembles monocyte-derived immature DC (iDC) 
      according to the expression of CD1a, CD83, and CCR7. The antimicrobial activity 
      of iDC against intracellular M. Tb inversely correlated with TNF-alpha-release 
      and was enhanced by treatment with anti-TNF-alpha Abs. Differentiation of iDC 
      into mature DC by addition of TNF-alpha or activation via Toll-like receptors 
      further reduced killing of M. Tb. The antibacterial activity against 
      intracellular M. Tb of all DCs was significantly lower than alveolar macrophages. 
      Therefore, the maintenance of a pool of DCs at the site of disease activity in 
      tuberculosis, and the maturation of these DC by TNF-alpha provides a mechanism by 
      which M. Tb escapes the innate immune system.
FAU - Buettner, Maike
AU  - Buettner M
AD  - Institut fur Klinische Mikrobiologie, Immunologie und Hygiene, Universitat 
      Erlangen-Nurnberg, Erlangen, Germany.
FAU - Meinken, Christoph
AU  - Meinken C
FAU - Bastian, Max
AU  - Bastian M
FAU - Bhat, Rauf
AU  - Bhat R
FAU - Stossel, Elmar
AU  - Stossel E
FAU - Faller, Gerhard
AU  - Faller G
FAU - Cianciolo, George
AU  - Cianciolo G
FAU - Ficker, Joachim
AU  - Ficker J
FAU - Wagner, Manfred
AU  - Wagner M
FAU - Rollinghoff, Martin
AU  - Rollinghoff M
FAU - Stenger, Steffen
AU  - Stenger S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Bacteria/growth & development/*immunology
MH  - Bronchoalveolar Lavage
MH  - *Cell Differentiation
MH  - Cells, Cultured
MH  - Dendritic Cells/*cytology/*immunology
MH  - Humans
MH  - *Immunity, Innate
MH  - Immunophenotyping
MH  - Macrophages, Alveolar/immunology
MH  - Mycobacterium tuberculosis/growth & development/immunology
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2005/03/22 09:00
MHDA- 2005/06/09 09:00
CRDT- 2005/03/22 09:00
PHST- 2005/03/22 09:00 [pubmed]
PHST- 2005/06/09 09:00 [medline]
PHST- 2005/03/22 09:00 [entrez]
AID - 174/7/4203 [pii]
AID - 10.4049/jimmunol.174.7.4203 [doi]
PST - ppublish
SO  - J Immunol. 2005 Apr 1;174(7):4203-9. doi: 10.4049/jimmunol.174.7.4203.