PMID- 15781451
OWN - NLM
STAT- MEDLINE
DCOM- 20050802
LR  - 20231213
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 280
IP  - 23
DP  - 2005 Jun 10
TI  - Nectin-like protein 2 defines a subset of T-cell zone dendritic cells and is a 
      ligand for class-I-restricted T-cell-associated molecule.
PG  - 21955-64
AB  - Dendritic cells (DCs) are a phenotypically and functionally heterogenous 
      population of leukocytes with distinct subsets serving a different set of 
      specialized immune functions. Here we applied an in vitro whole cell panning 
      approach using antibody phage display technology to identify cell-surface 
      epitopes specifically expressed on human blood BDCA3(+) DCs. A single-chain 
      antibody fragment (anti-1F12 scFv) was isolated that recognizes a conserved 
      surface antigen expressed on both human BDCA3(+) DCs and mouse CD8alpha(+) DCs. 
      We demonstrate that anti-1F12 scFv binds Nectin-like protein 2 (Necl2, Tslc1, 
      SynCaM, SgIGSF, or Igsf4), an adhesion molecule involved in tumor suppression, 
      synapse formation, and spermatogenesis. Thus, Necl2 defines a specialized subset 
      of DCs in both mouse and human. We further show that Necl2 binds 
      Class-I-restricted T-cell-associated molecule (CRTAM), a receptor primarily 
      expressed on activated cytotoxic lymphocytes. When present on antigen presenting 
      cells, Necl2 regulates IL-22 expression by activated CD8(+) T-cells. We propose 
      that Necl2/CRTAM molecular pair could regulate a large panel of cell/cell 
      interactions both within and outside of the immune system.
FAU - Galibert, Laurent
AU  - Galibert L
AD  - Department of Molecular Sciences, Amgen Inc., 1201 Amgen Court, Seattle, WA 
      98119-3105, USA.
FAU - Diemer, Geoffrey S
AU  - Diemer GS
FAU - Liu, Zhi
AU  - Liu Z
FAU - Johnson, Richard S
AU  - Johnson RS
FAU - Smith, Jeffrey L
AU  - Smith JL
FAU - Walzer, Thierry
AU  - Walzer T
FAU - Comeau, Michael R
AU  - Comeau MR
FAU - Rauch, Charles T
AU  - Rauch CT
FAU - Wolfson, Martin F
AU  - Wolfson MF
FAU - Sorensen, Rick A
AU  - Sorensen RA
FAU - Van der Vuurst de Vries, Anne-Renee
AU  - Van der Vuurst de Vries AR
FAU - Branstetter, Daniel G
AU  - Branstetter DG
FAU - Koelling, Raymond M
AU  - Koelling RM
FAU - Scholler, John
AU  - Scholler J
FAU - Fanslow, William C
AU  - Fanslow WC
FAU - Baum, Peter R
AU  - Baum PR
FAU - Derry, Jonathan M
AU  - Derry JM
FAU - Yan, Wei
AU  - Yan W
LA  - eng
PT  - Journal Article
DEP - 20050321
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Cadm1 protein, mouse)
RN  - 0 (Cell Adhesion Molecule-1)
RN  - 0 (Cell Adhesion Molecules)
RN  - 0 (Immunoglobulins)
RN  - 0 (Interleukins)
RN  - 0 (Ligands)
RN  - 0 (Membrane Proteins)
RN  - 0 (Peptide Library)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (class-I restricted T cell-associated molecule)
RN  - 9002-93-1 (Octoxynol)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Cell Adhesion
MH  - Cell Adhesion Molecule-1
MH  - Cell Adhesion Molecules
MH  - Cell Aggregation
MH  - Cell Line, Tumor
MH  - Cell Membrane/metabolism
MH  - Cell Separation
MH  - Coculture Techniques
MH  - Dendritic Cells/*cytology
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Immune System/physiology
MH  - Immunoblotting
MH  - Immunoglobulins/*metabolism/*physiology
MH  - Immunoprecipitation
MH  - Interleukins/biosynthesis
MH  - Lentivirus/genetics
MH  - Leukocytes/metabolism
MH  - Ligands
MH  - Lymphocytes/cytology
MH  - Mass Spectrometry
MH  - Membrane Proteins/metabolism/*physiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Octoxynol/pharmacology
MH  - Peptide Library
MH  - Phenotype
MH  - Polymerase Chain Reaction
MH  - Protein Binding
MH  - Protein Structure, Tertiary
MH  - RNA, Messenger/metabolism
MH  - Recombinant Fusion Proteins/metabolism
MH  - Spleen/metabolism
MH  - T-Lymphocytes/*metabolism
MH  - Interleukin-22
EDAT- 2005/03/23 09:00
MHDA- 2005/08/03 09:00
CRDT- 2005/03/23 09:00
PHST- 2005/03/23 09:00 [pubmed]
PHST- 2005/08/03 09:00 [medline]
PHST- 2005/03/23 09:00 [entrez]
AID - S0021-9258(20)69139-6 [pii]
AID - 10.1074/jbc.M502095200 [doi]
PST - ppublish
SO  - J Biol Chem. 2005 Jun 10;280(23):21955-64. doi: 10.1074/jbc.M502095200. Epub 2005 
      Mar 21.