PMID- 1578221
OWN - NLM
STAT- MEDLINE
DCOM- 19920610
LR  - 20190827
IS  - 0146-6615 (Print)
IS  - 0146-6615 (Linking)
VI  - 36
IP  - 4
DP  - 1992 Apr
TI  - Serologic differentiation of human T lymphotropic virus type I from type II 
      infection by synthetic peptide immunoassays.
PG  - 298-302
AB  - Synthetic peptide-based serologic assays (Select-HTLV and SynthEIA) that 
      distinguish the closely related human T-cell lymphotropic virus types I (HTLV-I) 
      and II (HTLV-II) were tested blindly for their ability to correctly identify 
      infection caused by either virus type. Of 57 HTLV-I and 38 HTLV-II specimens from 
      individuals whose infections were confirmed by polymerase chain reaction, the 
      Select-HTLV assay categorized 56 (98%) as HTLV-I, 36 (95%) as HTLV-II, and 3 (3%) 
      as nontypeable. Similarly, the SynthEIA assay categorized 54 (95%) as HTLV-I, 29 
      (76%) as HTLV-II, and 12 (13%) as nontypeable. More importantly, no specimen was 
      wrongly classified by either assay (100% specificity). Further, analysis of 
      serial specimens from six patients also demonstrated concordant results with the 
      PCR findings. Our results suggest that serotyping of HTLV infections can be 
      achieved reliably by these peptide assays, thus the need for technically complex 
      PCR-based HTLV typing, while not eliminated, can be greatly reduced.
FAU - Roberts, C R
AU  - Roberts CR
AD  - Department of Diagnostic Retrovirology, Walter Reed Army Institute of Research, 
      Washington, D.C.
FAU - Mitra, R
AU  - Mitra R
FAU - Hyams, K
AU  - Hyams K
FAU - Brodine, S K
AU  - Brodine SK
FAU - Lal, R B
AU  - Lal RB
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Med Virol
JT  - Journal of medical virology
JID - 7705876
RN  - 0 (Peptides)
SB  - IM
MH  - Base Sequence
MH  - Diagnosis, Differential
MH  - Enzyme-Linked Immunosorbent Assay
MH  - HTLV-I Infections/*diagnosis
MH  - HTLV-II Infections/*diagnosis
MH  - Human T-lymphotropic virus 1/*immunology
MH  - Human T-lymphotropic virus 2/*immunology
MH  - Humans
MH  - Molecular Sequence Data
MH  - Peptides/immunology
MH  - Polymerase Chain Reaction
MH  - Sensitivity and Specificity
MH  - Serologic Tests
EDAT- 1992/04/01 00:00
MHDA- 1992/04/01 00:01
CRDT- 1992/04/01 00:00
PHST- 1992/04/01 00:00 [pubmed]
PHST- 1992/04/01 00:01 [medline]
PHST- 1992/04/01 00:00 [entrez]
AID - 10.1002/jmv.1890360412 [doi]
PST - ppublish
SO  - J Med Virol. 1992 Apr;36(4):298-302. doi: 10.1002/jmv.1890360412.