PMID- 15782555
OWN - NLM
STAT- MEDLINE
DCOM- 20050411
LR  - 20161124
IS  - 1214-6994 (Print)
IS  - 1214-6994 (Linking)
VI  - 105
IP  - 3
DP  - 2004
TI  - Hypoxia-induced long-term increase of dopamine and tyrosine hydroxylase mRNA 
      levels.
PG  - 291-300
AB  - The aim of the present study was to determine hypoxia-induced changes in the 
      long-term expression of tyrosine hydroxylase (TH) mRNA and the steady-state 
      dopamine (DA) levels in rat mesencephalic cell cultures. The cultures were 
      exposed to hypoxia during the early developmental period, and DA content and TH 
      mRNA expression were determined on day in vitro (DIV) 14. Hypoxic exposure of 
      5-day-old cultures resulted in increased DA (control 89.9+/-8.9, hypoxia 
      135.8+/-23.7 pg/microg protein) and TH mRNA (control 37.3+/-4.7, hypoxia 
      143.1+/-49.4 pg/microg RNA) levels. To analyze the involvement of 
      hypoxia-inducible factor-1 (HIF-1) in these changes, we studied its activation 
      using reporter gene. Hypoxia caused a 3-fold increase in HIF-1 activity. Our data 
      suggest that hypoxia/ischemia during the putative critical developmental period 
      of neurons may determine the tyrosine hydroxylase gene expression and, 
      consequently, the development of the dopaminergic system.
FAU - Leclere, N
AU  - Leclere N
AD  - Department of Otorhinolaryngology, Charite Hospital, Berlin, Germany.
FAU - Andreeva, N
AU  - Andreeva N
FAU - Fuchs, J
AU  - Fuchs J
FAU - Kietzmann, T
AU  - Kietzmann T
FAU - Gross, J
AU  - Gross J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Czech Republic
TA  - Prague Med Rep
JT  - Prague medical report
JID - 101227436
RN  - 0 (DNA-Binding Proteins)
RN  - 0 (Hif1a protein, rat)
RN  - 0 (Hypoxia-Inducible Factor 1)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Nuclear Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transcription Factors)
RN  - EC 1.14.16.2 (Tyrosine 3-Monooxygenase)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - DNA-Binding Proteins/metabolism
MH  - Dopamine/genetics/*metabolism
MH  - Gene Expression
MH  - Hypoxia/*metabolism
MH  - Hypoxia-Inducible Factor 1
MH  - Hypoxia-Inducible Factor 1, alpha Subunit
MH  - Mesencephalon/*metabolism
MH  - Nuclear Proteins/metabolism
MH  - RNA, Messenger/*metabolism
MH  - Rats
MH  - Rats, Wistar
MH  - Transcription Factors/metabolism
MH  - Tyrosine 3-Monooxygenase/genetics/*metabolism
EDAT- 2005/03/24 09:00
MHDA- 2005/04/12 09:00
CRDT- 2005/03/24 09:00
PHST- 2005/03/24 09:00 [pubmed]
PHST- 2005/04/12 09:00 [medline]
PHST- 2005/03/24 09:00 [entrez]
PST - ppublish
SO  - Prague Med Rep. 2004;105(3):291-300.