PMID- 15782966
OWN - NLM
STAT- MEDLINE
DCOM- 20050705
LR  - 20191210
IS  - 0021-9673 (Print)
IS  - 0021-9673 (Linking)
VI  - 1065
IP  - 2
DP  - 2005 Feb 18
TI  - Separation and quantification of beer carbohydrates by high-performance liquid 
      chromatography with evaporative light scattering detection.
PG  - 207-10
AB  - An HPLC method with an evaporative light scattering detector was optimized and 
      validated for quantification of carbohydrates in beer. The chromatographic 
      separation was achieved using a Spherisorb NH2, 5 microm chromatographic column 
      and gradient elution with acetonitrile/water. The determinations were performed 
      in the linear range of 0.05-5.0 g/L for fructose, 0.05-5.0 g/L for glucose, 
      0.05-15.0 g/L for maltose, 0.05-10.0 g/L for maltotriose, and 0.05-5.0 g/L for 
      maltotetraose. The detection limits were 0.005 g/L for fructose, 0.008 g/L for 
      glucose, and 0.01 g/L for maltose, maltotriose, and maltotetraose. The 
      reliability of the method in terms of precision and accuracy was evaluated in 
      three beer matrices, low alcohol beer, 6% alcohol beer, and beer made with part 
      of adjuncts (4.5% alcohol). Relative standard deviations (RSDs) ranged between 
      1.59 and 5.95% (n = 10), and recoveries ranged between 94 and 98.4%.
FAU - Nogueira, Luciana C
AU  - Nogueira LC
AD  - Laboratorio de Bioquimica Nutricional e de Alimentos, Instituto de Quimica, 
      Universidade Federal do Rio de Janeiro, Brazil. luciana.nogueira@ig.com.br
FAU - Silva, Filipe
AU  - Silva F
FAU - Ferreira, Isabel M P L V O
AU  - Ferreira IM
FAU - Trugo, L C
AU  - Trugo LC
LA  - eng
PT  - Journal Article
PT  - Validation Study
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Carbohydrates)
SB  - IM
MH  - Beer/*analysis
MH  - Carbohydrates/*analysis
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Light
MH  - Reproducibility of Results
MH  - *Scattering, Radiation
EDAT- 2005/03/24 09:00
MHDA- 2005/07/06 09:00
CRDT- 2005/03/24 09:00
PHST- 2005/03/24 09:00 [pubmed]
PHST- 2005/07/06 09:00 [medline]
PHST- 2005/03/24 09:00 [entrez]
AID - S0021-9673(05)00027-0 [pii]
AID - 10.1016/j.chroma.2004.12.074 [doi]
PST - ppublish
SO  - J Chromatogr A. 2005 Feb 18;1065(2):207-10. doi: 10.1016/j.chroma.2004.12.074.