PMID- 15785374
OWN - NLM
STAT- MEDLINE
DCOM- 20050425
LR  - 20190713
IS  - 0041-1337 (Print)
IS  - 0041-1337 (Linking)
VI  - 79
IP  - 6
DP  - 2005 Mar 27
TI  - Treatment of streptozotocin-induced diabetes mellitus in rats by transplantation 
      of islet cells from two major histocompatibility complex disparate rats in 
      combination with intra bone marrow injection of allogeneic bone marrow cells.
PG  - 680-7
AB  - BACKGROUND: We have established a new method for the transplantation of 
      allogeneic pancreatic islets obtained from two different rat strains in 
      combination with a newly developed bone marrow transplantation (BMT) method in 
      which bone marrow cells (BMCs) are directly injected into the bone marrow cavity 
      (intra bone marrow BMT [IBM-BMT]). METHODS: Streptozotocin-induced diabetic Brown 
      Norway (BN: RT1A(n)) rats were injected with fludarabine, irradiated with 5.0 Gy 
      x 2, and BMCs from two allogeneic rat strains, Fischer 344 (F344: RT1A(1)) and 
      PVG (PVG: RT1A(c)), were then directly injected into the bone marrow cavity 
      (IBM-BMT). Simultaneously, approximately 600 pancreatic islets (PIs) from F344 
      and PVG rats were mixed and transplanted into the liver by way of the portal 
      vein. RESULTS: All the recipients thus treated showed normoglycemia 30 days after 
      the treatment. Hematolymphoid cells were completely reconstituted with the two 
      donor-type cells, and immunologic tolerance to F344 and PVG major 
      histocompatibility complex (MHC) determinants were induced. CONCLUSIONS: The 
      transplantation of PIs from two MHC-disparate donors was completely achieved in 
      combination with IBM-BMT, resulting in the improvement of blood glucose levels 
      and the amelioration of diabetes mellitus.
FAU - Taira, Mitsuru
AU  - Taira M
AD  - First Department of Pathology, Kansai Medical University, 10-15 Fumizono-cho, 
      Moriguchi City, Osaka 570-8506, Japan.
FAU - Inaba, Muneo
AU  - Inaba M
FAU - Takada, Keizo
AU  - Takada K
FAU - Baba, Susumu
AU  - Baba S
FAU - Fukui, Junichi
AU  - Fukui J
FAU - Ueda, Yusuke
AU  - Ueda Y
FAU - Kwon, A-Hon
AU  - Kwon AH
FAU - Hisha, Hiroko
AU  - Hisha H
FAU - Kamiyama, Yasuo
AU  - Kamiyama Y
FAU - Ikehara, Susumu
AU  - Ikehara S
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Transplantation
JT  - Transplantation
JID - 0132144
RN  - 0 (Antigens)
RN  - 0 (Blood Glucose)
RN  - 5W494URQ81 (Streptozocin)
SB  - IM
MH  - Animals
MH  - Antigens/immunology
MH  - Blood Glucose/metabolism
MH  - Bone Marrow Transplantation/*immunology
MH  - Diabetes Mellitus, Experimental/chemically induced/immunology/*pathology/*therapy
MH  - Glucose Tolerance Test
MH  - Graft Survival/immunology
MH  - Histocompatibility/*immunology
MH  - Islets of Langerhans/pathology
MH  - Islets of Langerhans Transplantation/*immunology
MH  - Lymphocyte Culture Test, Mixed
MH  - Rats
MH  - Streptozocin/*pharmacology
MH  - Time Factors
MH  - Tissue Donors
MH  - Transplantation Chimera/immunology
MH  - Transplantation, Homologous/immunology
EDAT- 2005/03/24 09:00
MHDA- 2005/04/26 09:00
CRDT- 2005/03/24 09:00
PHST- 2005/03/24 09:00 [pubmed]
PHST- 2005/04/26 09:00 [medline]
PHST- 2005/03/24 09:00 [entrez]
AID - 00007890-200503270-00013 [pii]
AID - 10.1097/01.tp.0000155500.17348.94 [doi]
PST - ppublish
SO  - Transplantation. 2005 Mar 27;79(6):680-7. doi: 10.1097/01.tp.0000155500.17348.94.