PMID- 15786508
OWN - NLM
STAT- MEDLINE
DCOM- 20050819
LR  - 20091211
IS  - 1552-4949 (Print)
IS  - 1552-4949 (Linking)
VI  - 65
IP  - 1
DP  - 2005 May
TI  - Post-reperfusion changes of monocyte function in coronary blood after 
      extracorporeal circulation.
PG  - 14-21
AB  - BACKGROUND: Neutrophil and mononuclear cell functional changes represent a 
      hallmark of inflammation during cardiopulmonary bypass and cardiovascular 
      surgery. Knowledge of mechanisms underlying monocyte functional modulation in 
      coronary blood may be useful to develop protective interventions that can limit 
      ischemia/reperfusion injury. METHODS: Samples of 13 patients were drawn from 
      venous coronary sinus before cardioplegic arrest and after reperfusion. The 
      following parameters were studied: surface molecules expression (CD18, CD11b, 
      CD44, CD162, CD15s, CD80, CD86, CD16, CD49d, CD29, CD25, HLA-DR, Toll-like 
      receptor-4 [TLR-4], CXCR1, CCR2, CCR5, CX3CR1), oxidative burst response, 
      monocyte-platelet conjugates (using antibodies against CD45, CD14, CD41a), and 
      platelet activation (CD62P, PAC-1). Enzyme-linked immunosorbent assays were 
      performed to measure levels of interleukin (IL)-1beta, IL-6, IL-8, IL-10, and 
      tumor necrosis factor-alpha (TNF-alpha). RESULTS: Coronary reperfusion 
      down-modulated monocyte molecules expression, especially for CD18 (P = 0.048), 
      CD44 (P = 0.0035), CD49d (P = 0.0029), CD29 (P = 0.032), HLA-DR (P < 0.0001), 
      TLR-4 (P = 0.0109), CCR2 (P = 0.0184), CCR5 (P = 0.0396), and CX3CR1 (P < 
      0.0001). A marginal increase (P = 0.062) of a normalized adhesion index between 
      monocytes and platelets was observed at reperfusion. No variations were detected 
      for the monocyte oxidative burst and platelet activation. Increased levels of 
      IL-6 (P = 0.013), TNF-alpha (P = 0.0272), and IL-10 (P = 0.0008) were measured 
      after cardioplegia. CONCLUSIONS: The lack of CD11b and CD25 variations and of the 
      oxidative burst exclude monocyte activation at reperfusion. The high 
      after-cardioplegia level of IL-10, the decreased expression of HLA-DR and TLR-4, 
      and the absence of IL-1beta and IL-8 suggest an IL-10-mediated functional 
      depression of monocyte, including their adhesive and migratory capacities. The 
      lack of an after-cardioplegia orientation toward IL-10 producing a 
      "macrophage-like" CD14+/CD16+ phenotype might mean that myocardial infiltrating 
      lymphocytes are the main source of IL-10. Moreover, the increased 
      after-cardioplegia levels of IL-6 and TNF-alpha might be due to myocardial and 
      endothelial activations. The increased adhesion index between monocyte and 
      platelets, without receptor variations, suggests a monocyte membrane modification 
      induced by extracorporeal circulation.
CI  - Copyright 2005 Wiley-Liss, Inc.
FAU - Sbrana, Silverio
AU  - Sbrana S
AD  - Laboratory of Hematology and Flow Cytometry, CNR Institute of Clinical 
      Physiology, Massa, Italy. sbrana@ifc.cnr.it
FAU - Bevilacqua, Stefano
AU  - Bevilacqua S
FAU - Buffa, Manuela
AU  - Buffa M
FAU - Spiller, Dario
AU  - Spiller D
FAU - Parri, Maria Serena
AU  - Parri MS
FAU - Gianetti, Jacopo
AU  - Gianetti J
FAU - De Filippis, Rossella
AU  - De Filippis R
FAU - Clerico, Aldo
AU  - Clerico A
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cytometry B Clin Cytom
JT  - Cytometry. Part B, Clinical cytometry
JID - 101235690
RN  - 0 (Antigens, Surface)
RN  - 0 (Interleukins)
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Antigens, Surface/biosynthesis
MH  - Cell Adhesion
MH  - Coronary Circulation
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Flow Cytometry
MH  - Gene Expression Regulation
MH  - Heart Arrest/blood
MH  - Humans
MH  - Interleukins/biosynthesis
MH  - Leukocytes, Mononuclear/cytology
MH  - Male
MH  - Middle Aged
MH  - Monocytes/cytology/*pathology
MH  - Neutrophils/cytology
MH  - Platelet Activation
MH  - Reperfusion
MH  - Reperfusion Injury/*prevention & control
MH  - Respiratory Burst
EDAT- 2005/03/24 09:00
MHDA- 2005/08/20 09:00
CRDT- 2005/03/24 09:00
PHST- 2005/03/24 09:00 [pubmed]
PHST- 2005/08/20 09:00 [medline]
PHST- 2005/03/24 09:00 [entrez]
AID - 10.1002/cyto.b.20049 [doi]
PST - ppublish
SO  - Cytometry B Clin Cytom. 2005 May;65(1):14-21. doi: 10.1002/cyto.b.20049.