PMID- 15786734
OWN - NLM
STAT- MEDLINE
DCOM- 20050901
LR  - 20190818
IS  - 0300-8177 (Print)
IS  - 0300-8177 (Linking)
VI  - 269
IP  - 1-2
DP  - 2005 Jan
TI  - Interaction of keratinocytes and fibroblasts modulates the expression of matrix 
      metalloproteinases-2 and -9 and their inhibitors.
PG  - 209-16
AB  - Disruption of epidermal-mesenchymal communication due to a delay in 
      epithelialization, increases the frequency of developing fibrotic conditions in 
      skin. As matrix metalloproteinases-2 (MMP-2) and -9 (MMP-9) are two key enzymes 
      involved in wound healing and tissue remodeling, here we examined the efficacy of 
      keratinocyte-fibroblast interaction on modulation of these enzymes and their 
      inhibitors. The conditioned media derived from keratinocytes and fibroblasts 
      grown in upper and lower chambers of a co-culture system, respectively, were 
      analyzed for MMP-2 and -9. Keratinocyte or fibroblast conditioned medium (FCM) 
      was used as a control. Gelatinolytic activity analyzed by zymography showed that 
      keratinocytes mainly express MMP-9 and to a lesser extent MMP-2; while 
      fibroblasts express only MMP-2. In a co-culture system, the activities of both 
      MMP-2 and MMP-9 markedly increased in conditioned media collected from bottom 
      chambers. These findings were consistent with the level of MMP-2 and MMP-9 
      measured by Western blot. Using the same experimental setting, the levels of 
      tissue inhibitors of MMPs (TIMPs) secreted by keratinocytes and fibroblasts grown 
      in the same co-culture system were also evaluated. Western blot showed that 
      fibroblasts secrete only TIMP-1 and TIMP-2 whose levels were increased by 
      co-culturing fibroblasts with keratinocytes. In contrary the level of TIMP-3, 
      which was mainly expressed by keratinocytes, increased by co-culturing these 
      cells with fibroblasts. In conclusion, interaction of fibroblast-keratinocyte 
      modulates the levels of MMP-2 and -9 and their inhibitors produced by these cells 
      and this interaction may be critical for a better healing quality at a late stage 
      of the wound healing process.
FAU - Sawicki, Grzegorz
AU  - Sawicki G
AD  - Department of Pharmacology, Cardiovascular Research Group, University of Alberta, 
      Edmonton, Alberta, Canada.
FAU - Marcoux, Yvonne
AU  - Marcoux Y
FAU - Sarkhosh, Kourosh
AU  - Sarkhosh K
FAU - Tredget, Edward E
AU  - Tredget EE
FAU - Ghahary, Aziz
AU  - Ghahary A
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 127497-59-0 (Tissue Inhibitor of Metalloproteinase-2)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - *Cell Communication
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Culture Media, Conditioned
MH  - Fibroblasts/*enzymology
MH  - Humans
MH  - Keratinocytes/*enzymology
MH  - Matrix Metalloproteinase 2/analysis/*metabolism
MH  - Matrix Metalloproteinase 9/analysis/*metabolism
MH  - Tissue Inhibitor of Metalloproteinase-1/analysis/metabolism
MH  - Tissue Inhibitor of Metalloproteinase-2/analysis/metabolism
MH  - Wound Healing/physiology
EDAT- 2005/03/25 09:00
MHDA- 2005/09/02 09:00
CRDT- 2005/03/25 09:00
PHST- 2005/03/25 09:00 [pubmed]
PHST- 2005/09/02 09:00 [medline]
PHST- 2005/03/25 09:00 [entrez]
AID - 10.1007/s11010-005-3178-x [doi]
PST - ppublish
SO  - Mol Cell Biochem. 2005 Jan;269(1-2):209-16. doi: 10.1007/s11010-005-3178-x.