PMID- 15792120
OWN - NLM
STAT- MEDLINE
DCOM- 20050628
LR  - 20050328
IS  - 0344-0338 (Print)
IS  - 0344-0338 (Linking)
VI  - 200
IP  - 11-12
DP  - 2005
TI  - Correlation of donor-derived keratinocytes and severity of graft-versus-host 
      disease (GVHD) in epidermis.
PG  - 775-81
AB  - Recent reports suggest that bone marrow might serve as a source of skin 
      progenitor cells. Graft-versus-host disease (GVHD) is a systemic disease that 
      involves the skin, the liver, and the gastrointestinal tract, and contributes to 
      transplant-related morbidity and mortality. To evaluate whether donor-derived 
      bone marrow cells participated in its pathophysiology, we correlated the severity 
      of GVHD in skin from sex-mismatched recipients with the percentage of 
      donor-derived keratinocytes using fluorescence in situ hybridization (FISH) for 
      detecting the Y chromosome. Y-positive signals were observed in female epidermis 
      in both keratinocytes and lymphocytes. These results support the notion that 
      donor-derived hematopoietic stem cells may contain pluripotent stem cells. 
      Furthermore, there was a strong correlation between the frequency of Y-positive 
      keratinocytes and the histopathologic grade of GVHD (p = 0.004), as well as with 
      the number of Y-positive lymphocytes infiltrating the epidermis (p = 0.005), 
      suggesting a role for donor-derived keratinocytes in the pathophysiology of GVHD. 
      However, no clusters of Y-positive keratinocytes were found in the epidermis, and 
      no correlation was observed between the number of Y-positive keratinocytes and 
      time course of GVHD after transplantation. We hypothesize that donor-derived stem 
      cells may play a role in the regeneration of damaged keratinocytes in GVHD.
FAU - Niino, Daisuke
AU  - Niino D
AD  - Department of Molecular Pathology, Atomic Bomb Disease Institute, Nagasaki 
      University Graduate School of Biomedical Sciences, Nagasaki, Japan.
FAU - Nakashima, Masahiro
AU  - Nakashima M
FAU - Kondo, Hisayoshi
AU  - Kondo H
FAU - Ito, Masahiro
AU  - Ito M
FAU - Matsuu, Mutsumi
AU  - Matsuu M
FAU - Shichijo, Kazuko
AU  - Shichijo K
FAU - Nakayama, Toshiyuki
AU  - Nakayama T
FAU - Tomonaga, Masao
AU  - Tomonaga M
FAU - Sekine, Ichiro
AU  - Sekine I
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - Pathol Res Pract
JT  - Pathology, research and practice
JID - 7806109
SB  - IM
MH  - Adult
MH  - Bone Marrow Transplantation/*pathology
MH  - Cell Count
MH  - Chromosomes, Human, Y/genetics
MH  - Epidermis/*pathology
MH  - Female
MH  - Graft vs Host Disease/etiology/genetics/*pathology
MH  - *Graft vs Host Reaction
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Keratinocytes/*pathology
MH  - Middle Aged
MH  - Pluripotent Stem Cells/pathology/physiology
MH  - Skin Diseases/etiology/genetics/*pathology
MH  - Tissue Donors
EDAT- 2005/03/29 09:00
MHDA- 2005/06/29 09:00
CRDT- 2005/03/29 09:00
PHST- 2005/03/29 09:00 [pubmed]
PHST- 2005/06/29 09:00 [medline]
PHST- 2005/03/29 09:00 [entrez]
AID - S0344-0338(04)00178-5 [pii]
AID - 10.1016/j.prp.2004.09.007 [doi]
PST - ppublish
SO  - Pathol Res Pract. 2005;200(11-12):775-81. doi: 10.1016/j.prp.2004.09.007.