PMID- 15792251
OWN - NLM
STAT- MEDLINE
DCOM- 20060725
LR  - 20061115
IS  - 1615-9306 (Print)
IS  - 1615-9306 (Linking)
VI  - 28
IP  - 4
DP  - 2005 Mar
TI  - Qualitative and quantitative analysis of active flavonoids in Flos Lonicerae by 
      capillary zone electrophoresis coupled with solid-phase extraction.
PG  - 365-72
AB  - Flavonoids are an important bioactive group in the commonly used herbal medicine 
      Flos Lonicerae. A new method of capillary zone electrophoresis (CZE) coupled with 
      solid-phase extraction (SPE) was developed for simultaneous assay of flavonoid 
      aglycones and glycosides in Flos Lonicerae. Optimum CZE separation was achieved 
      with a background electrolyte (BGE) solution consisting of 80 mM boric acid and 
      20 mM phosphate acid, adjusted to pH 8.1, with 15% acetonitrile (v/v) added, and 
      applying a separation voltage of 28 kV. The SPE method was used for pretreating 
      the complex matrix of botanical materials and good reproducibility was obtained 
      when avicularin was used as internal standard. Linearity of the method was 
      excellent with correlation coefficients (r2) in the range of 0.9995-0.9999 and 
      detection limits were lower than 0.6 microg/mL for the four flavonoids. The 
      obtained recoveries varied between 93 to 104% while the relative standard 
      deviations (RSDs) were below 4.4% (n=3). The developed CZE method was 
      successfully used for the separation of eight flavonoids and the quantification 
      of the four flavonoids in five species of Flos Lonicerae.
FAU - Chen, Jun
AU  - Chen J
AD  - Key Laboratory of Modern Chinese Medicines, Department of Pharmacognosy, China 
      Pharmaceutical University, Nanjing, People's Republic of China.
FAU - Li, Song-Lin
AU  - Li SL
FAU - Li, Ping
AU  - Li P
FAU - Song, Yue
AU  - Song Y
FAU - Chai, Xing-Yun
AU  - Chai XY
FAU - Ma, Ding-Yuan
AU  - Ma DY
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - J Sep Sci
JT  - Journal of separation science
JID - 101088554
RN  - 0 (Flavonoids)
SB  - IM
MH  - Animals
MH  - Electrophoresis, Capillary
MH  - Flavonoids/*analysis/*chemistry
MH  - Lepidoptera/*chemistry
MH  - Molecular Structure
MH  - Time Factors
EDAT- 2005/03/29 09:00
MHDA- 2006/07/26 09:00
CRDT- 2005/03/29 09:00
PHST- 2005/03/29 09:00 [pubmed]
PHST- 2006/07/26 09:00 [medline]
PHST- 2005/03/29 09:00 [entrez]
AID - 10.1002/jssc.200400024 [doi]
PST - ppublish
SO  - J Sep Sci. 2005 Mar;28(4):365-72. doi: 10.1002/jssc.200400024.