PMID- 15792355
OWN - NLM
STAT- MEDLINE
DCOM- 20050711
LR  - 20190818
IS  - 0300-8177 (Print)
IS  - 0300-8177 (Linking)
VI  - 270
IP  - 1-2
DP  - 2005 Feb
TI  - Energy metabolism in the granulation tissue of diabetic rats during cutaneous 
      wound healing.
PG  - 71-7
AB  - The skin cells chiefly depend on carbohydrate metabolism for their energy 
      requirement during cutaneous wound healing. Since the glucose metabolism is 
      greatly hampered in diabetes and this might affect wound repair process. This 
      prompted us to investigate the intermediate steps of energy metabolism by 
      measuring enzyme activities in the wound tissues of normal and 
      streptozotocin-induced diabetic rats following excision-type of cutaneous injury. 
      The activities of key regulatory enzymes namely hexokinase (HK), 
      phosphofructokinase (PFK), lactate dehydrogenase (LDH), citrate synthase (CS) and 
      glucose-6 phosphate dehydrogenase (G6PD) have been monitored in the granulation 
      tissues of normal and diabetic rats at different time points (2, 7, 14 and 21 
      days) of postwounding. Interestingly, a significant alteration in all these 
      enzyme activities was observed in diabetic rats. The activity of PFK was 
      increased but HK, LDH and CS showed a decreased activity in the wound tissue of 
      diabetics as compared to normal rats. However G6PD exhibited an elevated activity 
      only at early stage of healing in diabetic rats. Thus, the results suggest that 
      significant alterations in the activities of energy metabolizing enzymes in the 
      wound tissue of diabetic rats may affect the energy availability for cellular 
      activity needed for repair process and this may perhaps be one of the factor 
      responsible for impaired healing in these subjects.
FAU - Gupta, Asheesh
AU  - Gupta A
AD  - Division of Pharmacology, Central Drug Research Institute, Lucknow, India.
FAU - Raghubir, Ram
AU  - Raghubir R
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - Mol Cell Biochem
JT  - Molecular and cellular biochemistry
JID - 0364456
RN  - 5W494URQ81 (Streptozocin)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 1.1.1.49 (Glucosephosphate Dehydrogenase)
RN  - EC 2.3.3.1 (Citrate (si)-Synthase)
RN  - EC 2.7.1 - (Phosphofructokinases)
RN  - EC 2.7.1.1 (Hexokinase)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Citrate (si)-Synthase/metabolism
MH  - *Diabetes Mellitus, Experimental
MH  - Energy Metabolism
MH  - Glucose/metabolism
MH  - Glucosephosphate Dehydrogenase/metabolism
MH  - Granulation Tissue/*enzymology
MH  - Hexokinase/metabolism
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Male
MH  - Phosphofructokinases/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Skin/metabolism
MH  - Streptozocin/pharmacology
MH  - Time Factors
MH  - *Wound Healing
EDAT- 2005/03/29 09:00
MHDA- 2005/07/12 09:00
CRDT- 2005/03/29 09:00
PHST- 2005/03/29 09:00 [pubmed]
PHST- 2005/07/12 09:00 [medline]
PHST- 2005/03/29 09:00 [entrez]
AID - 10.1007/s11010-005-5258-3 [doi]
PST - ppublish
SO  - Mol Cell Biochem. 2005 Feb;270(1-2):71-7. doi: 10.1007/s11010-005-5258-3.