PMID- 15793719
OWN - NLM
STAT- MEDLINE
DCOM- 20060809
LR  - 20181201
IS  - 1531-5037 (Electronic)
IS  - 0022-3468 (Linking)
VI  - 40
IP  - 3
DP  - 2005 Mar
TI  - Altered chemokine response in an animal model of multiple organ dysfunction 
      syndrome induced by zymosan.
PG  - 464-9
AB  - PURPOSE: The aim of this study was to determine the monocyte chemoattractant 
      protein-1 (MCP-1) response over time in an animal model of multiple organ 
      dysfunction syndrome (MODS). METHODS: On day 0, rats were randomized to receive 
      an intraperitoneal injection of zymosan at a dose of 1 mg/g of body weight (n = 
      36) or vehicle (n = 9). Serum, peritoneal lavage (PL) fluid, and bronchoalveolar 
      lavage (BAL) fluid were collected from 3 rats in the control group and 6 to 7 
      rats in the zymosan group at days 1, 5, and 12. Monocyte chemoattractant 
      protein-1 concentrations were determined by enzyme-linked immunosorbent assay. 
      RESULTS: The authors observed a 47% mortality in the zymosan-treated rats. 
      Monocyte chemoattractant protein-1 levels were unchanged in the serum, PL, and 
      BAL of control animals. Both serum and PL MCP-1 were significantly higher in 
      zymosan-treated rats on days 1 (P < .01) and 5 (P < .05) when compared with 
      controls. By day 12, no difference between the 2 groups was observed. No 
      significant difference was noted in BAL MCP-1. CONCLUSIONS: Chemokines are 
      increased systemically and locally during MODS. The fact that MCP-1 is 
      significantly higher early in the course of MODS may suggest that this chemokine 
      is important in the early inflammatory changes that lead to MODS later in the 
      course of this illness.
FAU - Thomas, Neal J
AU  - Thomas NJ
AD  - Department of Pediatrics, Pediatric Critical Care Medicine, Penn State Children's 
      Hospital, Penn State University College of Medicine, Hershey, PA 17033, USA. 
      nthomas@psu.edu
FAU - Umstead, Todd M
AU  - Umstead TM
FAU - Phelps, David S
AU  - Phelps DS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Pediatr Surg
JT  - Journal of pediatric surgery
JID - 0052631
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemokines)
RN  - 9010-72-4 (Zymosan)
SB  - IM
MH  - Animals
MH  - Ascitic Fluid/chemistry
MH  - Bronchoalveolar Lavage Fluid/chemistry
MH  - Chemokine CCL2/analysis/*metabolism
MH  - Chemokines/*metabolism
MH  - Disease Models, Animal
MH  - Injections, Intraperitoneal
MH  - Male
MH  - Multiple Organ Failure/chemically induced/*metabolism
MH  - Peritonitis/chemically induced/physiopathology
MH  - Random Allocation
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Zymosan/administration & dosage/*toxicity
EDAT- 2005/03/29 09:00
MHDA- 2006/08/10 09:00
CRDT- 2005/03/29 09:00
PHST- 2005/03/29 09:00 [pubmed]
PHST- 2006/08/10 09:00 [medline]
PHST- 2005/03/29 09:00 [entrez]
AID - S0022346804008139 [pii]
AID - 10.1016/j.jpedsurg.2004.11.042 [doi]
PST - ppublish
SO  - J Pediatr Surg. 2005 Mar;40(3):464-9. doi: 10.1016/j.jpedsurg.2004.11.042.