PMID- 15794488
OWN - NLM
STAT- MEDLINE
DCOM- 20060111
LR  - 20190922
IS  - 0920-5063 (Print)
IS  - 0920-5063 (Linking)
VI  - 16
IP  - 2
DP  - 2005
TI  - Monocyte activation on polyelectrolyte multilayers.
PG  - 237-51
AB  - The adherence and activation of primary human monocytes was investigated on a 
      polyelectrolyte multilayer film containing hyaluronic acid (HA) and poly-L-lysine 
      (PLL). The sequential layer-by-layer deposition of the multilayer film was 
      characterized by surface plasmon resonance. Eight alternating bilayers displayed 
      an effective thickness of 16.15 nm with a total polymer coverage of 2.10 
      microg/cm2. For cell studies, HA-PLL multilayers were constructed on tissue 
      culture polystyrene (TCPS) substrates and characterized by time of flight second 
      ion mass spectrometry (ToF-SIMS) analysis. Principal component analysis of the 
      ToF-SIMS spectra resolved no significant difference in surface chemistry between 
      PLL-terminated and HA-terminated multilayer surfaces. Monocyte adhesion on PLL- 
      and HA-terminated surfaces was measured by the lactate dehydrogenase assay and 
      showed a significant decrease in cell adhesion after 24 h incubation. Cell 
      viability measured by Live/Dead fluorescent staining showed significant cell 
      death in the adherent cell population over these 24 h. Tumor necrosis 
      factor-alpha (TNF-alpha) production, a measure of monocyte activation, was 
      quantified by ELISA and normalized to the number of adherent monocytes. The 
      activation of monocytes on PLL-terminated and HA-terminated surfaces was nearly 
      identical, and both surfaces had TNF-alpha levels that were 8-fold higher than 
      TCPS. These results demonstrate that sufficient PLL had diffused into the surface 
      layer to direct monocyte adherence and to induce cytokine activation and cell 
      death on the HA-terminated multilayer films. The diffusion of the second 
      multilayer component to the coating surface should, thus, be taken into account 
      in the design of polyelectrolyte-based biomaterial coating strategies.
FAU - Hwang, Jason J
AU  - Hwang JJ
AD  - Department of Bioengineering, University of Washington, Box 351721, Seattle, WA 
      98195-1721, USA.
FAU - Jelacic, Sandra
AU  - Jelacic S
FAU - Samuel, Newton T
AU  - Samuel NT
FAU - Maier, Ronald V
AU  - Maier RV
FAU - Campbell, Charles T
AU  - Campbell CT
FAU - Castner, David G
AU  - Castner DG
FAU - Hoffman, Allan S
AU  - Hoffman AS
FAU - Stayton, Patrick S
AU  - Stayton PS
LA  - eng
GR  - EB 002027/EB/NIBIB NIH HHS/United States
GR  - R24-HL64387/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - J Biomater Sci Polym Ed
JT  - Journal of biomaterials science. Polymer edition
JID - 9007393
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 25104-18-1 (Polylysine)
RN  - 9004-61-9 (Hyaluronic Acid)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
SB  - IM
MH  - Cell Adhesion
MH  - Cell Survival
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Humans
MH  - Hyaluronic Acid/*chemistry
MH  - In Vitro Techniques
MH  - L-Lactate Dehydrogenase/metabolism
MH  - *Lymphocyte Activation
MH  - Mass Spectrometry
MH  - Monocytes/*physiology
MH  - Polylysine/*chemistry
MH  - Tumor Necrosis Factor-alpha/metabolism
EDAT- 2005/03/30 09:00
MHDA- 2006/01/13 09:00
CRDT- 2005/03/30 09:00
PHST- 2005/03/30 09:00 [pubmed]
PHST- 2006/01/13 09:00 [medline]
PHST- 2005/03/30 09:00 [entrez]
AID - 10.1163/1568562053115480 [doi]
PST - ppublish
SO  - J Biomater Sci Polym Ed. 2005;16(2):237-51. doi: 10.1163/1568562053115480.