PMID- 15795076
OWN - NLM
STAT- MEDLINE
DCOM- 20050816
LR  - 20081121
IS  - 0378-1135 (Print)
IS  - 0378-1135 (Linking)
VI  - 107
IP  - 1-2
DP  - 2005 Apr 25
TI  - New insights into the genetic diversity of European porcine reproductive and 
      respiratory syndrome virus (PRRSV).
PG  - 31-48
AB  - The complete ORF5 sequences of 66 porcine reproductive and respiratory syndrome 
      (PRRS) field virus strains (1991-2001) and three European modified live vaccine 
      strains were determined, as well as ORFs 6 and 7 of 19 selected strains. The 
      variability of the deduced ORF5 amino acid sequences was analysed using 
      statistical process control (SPC), allowing for the objective assessment of 
      variable and conserved regions. Four variable and four conserved regions as well 
      as five hypervariable amino acid positions were defined. The effects of genetic 
      variability on possible structural and functional properties were discussed with 
      emphasis on immunogenic features. Phylogenetic analysis and pairwise comparison 
      of the nucleotide sequences revealed that the genetic distances between the 
      strains has greatly increased over time. The data do not support an evolutionary 
      influence of the geographical location or the time of sample collection, nor of 
      PRRSV vaccination on strain development. In contrast to other authors who tended 
      to concentrate on the samples from either a common geographic origin or a short 
      sampling period, we could not confirm geographically separate PRRSV clusters nor 
      did we find evidence of positive selective pressure as measured by the ratio of 
      synonymous to non-synonymous substitutions in ORF5, 6 or 7. Immunological 
      implications and vaccination strategies are discussed.
FAU - Pesch, S
AU  - Pesch S
AD  - Bioscreen European Veterinary Disease Management Center GmbH, Mendelstr. 11, 
      48149 Munster, Germany.
FAU - Meyer, C
AU  - Meyer C
FAU - Ohlinger, V F
AU  - Ohlinger VF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Vaccines, Attenuated)
RN  - 0 (Viral Envelope Proteins)
RN  - 0 (Viral Vaccines)
RN  - 0 (glycoprotein 5, PRRSV)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cluster Analysis
MH  - Genes, Viral
MH  - *Genetic Variation
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - Porcine Reproductive and Respiratory Syndrome/prevention & control/*virology
MH  - Porcine respiratory and reproductive syndrome 
      virus/classification/*genetics/isolation & purification
MH  - Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Sequence Alignment/veterinary
MH  - Sequence Homology, Amino Acid
MH  - Swine
MH  - Vaccines, Attenuated/immunology
MH  - Viral Envelope Proteins/chemistry/genetics
MH  - Viral Vaccines/immunology
EDAT- 2005/03/30 09:00
MHDA- 2005/08/17 09:00
CRDT- 2005/03/30 09:00
PHST- 2004/09/24 00:00 [received]
PHST- 2005/01/19 00:00 [revised]
PHST- 2005/01/20 00:00 [accepted]
PHST- 2005/03/30 09:00 [pubmed]
PHST- 2005/08/17 09:00 [medline]
PHST- 2005/03/30 09:00 [entrez]
AID - S0378-1135(05)00039-8 [pii]
AID - 10.1016/j.vetmic.2005.01.028 [doi]
PST - ppublish
SO  - Vet Microbiol. 2005 Apr 25;107(1-2):31-48. doi: 10.1016/j.vetmic.2005.01.028.