PMID- 15796308
OWN - NLM
STAT- MEDLINE
DCOM- 20050920
LR  - 20190922
IS  - 0920-5063 (Print)
IS  - 0920-5063 (Linking)
VI  - 16
IP  - 1
DP  - 2005
TI  - Quantification of initial adhesion of Enterococcus faecalis to medical grade 
      polymers using a DNA-based fluorescence assay.
PG  - 115-29
AB  - This paper reports on the use of a DNA-based fluorescence assay to study and 
      quantify the initial interactions of the uropathogen Enterococcus faecalis with 
      different polymers commonly used for the fabrication of medical devices and 
      implants, including polyurethane (PU), silicone (SI), high-density polyethylene 
      (HDPE), polyamide (PA), poly(methyl methacrylate) (PMMA) and 
      polytetrafluoroethylene (PTFE). To follow the kinetics of E. faecalis adhesion, 
      polymer samples were incubated in bacterial solution for various times and the 
      relative concentration of adhered bacteria was obtained using two methods: 
      commonly used CFU plate counting and a DNA quantification assay. Results obtained 
      from DNA-based fluorescence assays showed that E. faecalis adhesion on PU is 
      3-times higher than that on PTFE following exposure to bacteria for 180 min. 
      Neither surface wettability nor surface roughness of the studied polymers was 
      found to correlate with E. faecalis adhesion, suggesting the involvement of much 
      more complex adhesion mechanisms of bacteria onto surfaces. SEM micrographs of 
      adhered bacteria illustrated that adhesion was different depending on the type of 
      polymeric substrate: adhesion on PU samples was characterized by the aggregation 
      of bacterial cells in dense clusters, as well as by the presence of fimbriae 
      between cells and the substrate, which could explain the high adhesion to PU 
      compared to the other polymers. This work demonstrated that the bacterial 
      adhesion to polymers occurs at an early stage of the contact and suggests that 
      the initial adhesion stage should be controlled, in order to prevent subsequent 
      biofilm formation and, thus, reduce the risk of implant-associated infections.
FAU - Senechal, Annie
AU  - Senechal A
AD  - Department of Biomedical Engineering, McGill University, 3775 University Street, 
      Montreal, Quebec, Canada H3A 2B4.
FAU - Catuogno, Christelle J
AU  - Catuogno CJ
FAU - Tabrizian, Maryam
AU  - Tabrizian M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Biomater Sci Polym Ed
JT  - Journal of biomaterials science. Polymer edition
JID - 9007393
RN  - 0 (Polymers)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - *Bacterial Adhesion
MH  - DNA/*metabolism
MH  - Enterococcus faecalis/*cytology/*metabolism/ultrastructure
MH  - Fluorescence
MH  - Fluorometry/*methods
MH  - Microscopy, Atomic Force
MH  - Microscopy, Electron, Scanning
MH  - Molecular Structure
MH  - Polymers/chemistry/*metabolism
EDAT- 2005/03/31 09:00
MHDA- 2005/09/21 09:00
CRDT- 2005/03/31 09:00
PHST- 2005/03/31 09:00 [pubmed]
PHST- 2005/09/21 09:00 [medline]
PHST- 2005/03/31 09:00 [entrez]
AID - 10.1163/1568562052843311 [doi]
PST - ppublish
SO  - J Biomater Sci Polym Ed. 2005;16(1):115-29. doi: 10.1163/1568562052843311.