PMID- 15796957
OWN - NLM
STAT- MEDLINE
DCOM- 20050509
LR  - 20061115
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 158
IP  - 2
DP  - 2005 Apr 15
TI  - Characterization of quantitative chromosomal abnormalities in renal cell 
      carcinomas by interphase four-color fluorescence in situ hybridization.
PG  - 110-8
AB  - Renal cell carcinomas (RCC) in adults are histologically heterogeneous solid 
      tumors with specific chromosomal abnormality patterns included in the World 
      Health Organization (WHO) classification. To overcome some of the drawbacks of 
      cytogenetic and comparative genomic hybridization (CGH) analyses, we designed a 
      first-generation cytogenetic diagnostic test using four-color fluorescence in 
      situ hybridization (FISH) on interphase nuclei. We selected 51 bacterial 
      artificial chromosome and P1-derived artificial chromosome clones covering 17 
      chromosomal regions involved in the abnormalities of the adult RCC histologic 
      subtypes. An initial set of probes allowed the identification of clear-cell RCC, 
      papillary RCC, and other RCC on a single slide. A second test allowed the 
      detection of additional chromosomal abnormalities or aberrations specific to 
      chromophobic RCC and oncocytomas. We tested 25 cases of RCC, and the results were 
      in agreement with those of cytogenetic techniques and/or CGH methods. The 
      techniques appeared to be very sensitive, because small tumoral cell clones that 
      were undetected by other cytogenetic methods were identified with this method. It 
      was concluded that the multicolor FISH test was specific and sensitive, easy to 
      perform, and could be part of the investigation process in RCC.
FAU - Receveur, Aline Ossard
AU  - Receveur AO
AD  - Laboratoire de Cytogenomique des cancers CNRS UMR 8125, Institut Gustave Roussy, 
      39 rue Camille Desmoulins 94805, Villejuif cedex 5, France.
FAU - Couturier, Jerome
AU  - Couturier J
FAU - Molinie, Vincent
AU  - Molinie V
FAU - Vieillefond, Annick
AU  - Vieillefond A
FAU - Desangles, Francois
AU  - Desangles F
FAU - Guillaud-Bataille, Marine
AU  - Guillaud-Bataille M
FAU - Danglot, Gisele
AU  - Danglot G
FAU - Coullin, Philippe
AU  - Coullin P
FAU - Bernheim, Alain
AU  - Bernheim A
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma, Renal Cell/classification/diagnosis/*genetics/pathology/surgery
MH  - *Chromosome Aberrations
MH  - Chromosome Deletion
MH  - Clone Cells/pathology
MH  - Cytogenetic Analysis
MH  - Diagnosis, Differential
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - *Interphase
MH  - Karyotyping
MH  - Kidney Neoplasms/classification/diagnosis/*genetics/pathology/surgery
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - Sensitivity and Specificity
MH  - Translocation, Genetic
MH  - Tumor Cells, Cultured
EDAT- 2005/03/31 09:00
MHDA- 2005/05/10 09:00
CRDT- 2005/03/31 09:00
PHST- 2004/06/09 00:00 [received]
PHST- 2004/08/09 00:00 [revised]
PHST- 2004/08/10 00:00 [accepted]
PHST- 2005/03/31 09:00 [pubmed]
PHST- 2005/05/10 09:00 [medline]
PHST- 2005/03/31 09:00 [entrez]
AID - S0165-4608(04)00338-3 [pii]
AID - 10.1016/j.cancergencyto.2004.08.019 [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 2005 Apr 15;158(2):110-8. doi: 
      10.1016/j.cancergencyto.2004.08.019.