PMID- 15797478
OWN - NLM
STAT- MEDLINE
DCOM- 20050524
LR  - 20061115
IS  - 0165-2427 (Print)
IS  - 0165-2427 (Linking)
VI  - 105
IP  - 1-2
DP  - 2005 May 1
TI  - Measurement of serum immunoglobulin E (IgE) specific for house dust mite antigens 
      in normal cats and cats with allergic skin disease.
PG  - 85-93
AB  - The purpose of this study was to determine whether cats with allergic skin 
      disease have significant concentrations of serum Immunoglobulin E (IgE) specific 
      for antigens derived from the house dust mites (HDM) Dermatophagoides farinae 
      (DF) and Dermatophagoides pteronyssinus (DP). Enzyme-linked immunosorbent assays 
      (ELISA) were developed for this purpose. Binding of serum allergen-specific IgE 
      was detected via the use of biotinylated Fc-epsilon receptor alpha chain protein 
      (FcvarepsilonRIalpha). Following optimisation of the assay, serum samples from 59 
      cats with allergic skin disease and 54 clinically normal cats were screened. 
      Results were expressed as ELISA units per ml (EU/ml) compared to a standard 
      curve. Serological findings were correlated with the clinical presentation of 
      affected cats. Cats with symptoms of feline allergic skin disease were grouped as 
      follows: self-induced alopecia without lesions (group 1), papulocrusting 
      dermatitis (group 2), eosinophilic granuloma complex (group 3), 
      papular/ulcerative dermatitis of head and neck/facial dermatitis (group 4), and a 
      combination of symptoms (group 5). Control normal cats comprised the final group 
      (group 6). The Kruskal-Wallis test was used for statistical analysis. There was 
      no significant difference between groups for DF- and DP-specific IgE 
      concentrations with a p-value of 0.875 and 0.705, respectively. Although the 
      FcvarepsilonRIalpha-based ELISA was able to detect house dust mite-specific 
      feline IgE, the presence of this allergen-specific IgE correlates poorly with the 
      presence of clinical manifestations of allergic skin disease. The results of this 
      study question the clinical relevance of house dust mite-specific IgE in feline 
      allergic skin disease.
FAU - Taglinger, K
AU  - Taglinger K
AD  - School of Clinical Veterinary Science, University of Bristol, Langford House, 
      Langford, Bristol BS40 5DU, UK.
FAU - Helps, C R
AU  - Helps CR
FAU - Day, M J
AU  - Day MJ
FAU - Foster, A P
AU  - Foster AP
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Vet Immunol Immunopathol
JT  - Veterinary immunology and immunopathology
JID - 8002006
RN  - 0 (Antigens, Dermatophagoides)
RN  - 0 (Receptors, IgE)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Antigens, Dermatophagoides/*immunology
MH  - Cat Diseases/*immunology
MH  - Cats/*immunology
MH  - Enzyme-Linked Immunosorbent Assay/methods/veterinary
MH  - Female
MH  - Immunoglobulin E/*blood
MH  - Male
MH  - Pyroglyphidae/*immunology
MH  - Receptors, IgE/immunology
MH  - Skin Diseases, Eczematous/immunology/*veterinary
MH  - Statistics, Nonparametric
EDAT- 2005/03/31 09:00
MHDA- 2005/05/25 09:00
CRDT- 2005/03/31 09:00
PHST- 2004/04/01 00:00 [received]
PHST- 2004/11/19 00:00 [revised]
PHST- 2004/12/16 00:00 [accepted]
PHST- 2005/03/31 09:00 [pubmed]
PHST- 2005/05/25 09:00 [medline]
PHST- 2005/03/31 09:00 [entrez]
AID - S0165-2427(04)00368-X [pii]
AID - 10.1016/j.vetimm.2004.12.017 [doi]
PST - ppublish
SO  - Vet Immunol Immunopathol. 2005 May 1;105(1-2):85-93. doi: 
      10.1016/j.vetimm.2004.12.017.