PMID- 15800872
OWN - NLM
STAT- MEDLINE
DCOM- 20050930
LR  - 20061115
IS  - 1615-9853 (Print)
IS  - 1615-9853 (Linking)
VI  - 5
IP  - 5
DP  - 2005 Apr
TI  - Changes in the proteomic profile during differentiation and maturation of human 
      monocyte-derived dendritic cells stimulated with granulocyte macrophage colony 
      stimulating factor/interleukin-4 and lipopolysaccharide.
PG  - 1186-98
AB  - Dendritic cells (DCs) are highly specialized antigen-presenting cells that play 
      an essential role in the immune response. We used the proteomic approach based on 
      two-dimensional gel electrophoresis and mass spectrometry to identify the protein 
      changes that occur during differentiation of DCs from monocytes (Mo) stimulated 
      with granulocyte macrophage colony stimulating factor/interleukin-4 (GM-CSF/IL-4) 
      and during the maturation of immature DCs stimulated with lipopolysaccharide. 
      Sixty-three differentially expressed proteins (+/- two-fold) were unambiguously 
      identified with sequence coverage greater than 20%. They corresponded to only 36 
      different proteins, because 11 were present as 38 electrophoretic forms. Some 
      proteins such as tropomyosin 4 and heat shock protein 71 presented differentially 
      expressed electrophoretic forms, suggesting that many of the changes in protein 
      expression that accompany differentiation and maturation of DCs occur in 
      post-translationally modified proteins. The largest differences in expression 
      were observed for actin (21-fold in Mo), Rho GDP-dissociation inhibitor 2 
      (20-fold in Mo), vimentin (eight-fold in immature DCs), lymphocyte-specific 
      protein 1 (12-fold in mature DCs) and thioredoxin (14-fold in mature DCs). 
      Several proteins are directly related to functional and morphological 
      characteristics of DCs, such as cytoskeletal proteins (cytoskeleton 
      rearrangement) and chaperones (antigen processing and presentation), but other 
      proteins have not been assigned specific functions in DCs. Only a few proteins 
      identified here were the same as those reported in proteomic studies of DCs, 
      which used different stimuli to produce the cells (GM-CSF/IL-4 and tumor necrosis 
      factor-alpha). These data suggest that the DC protein profile depends on the 
      stimuli used for differentiation and especially for maturation.
FAU - Pereira, Sandra Rodrigues
AU  - Pereira SR
AD  - Departamento de Bioquimica, Escola Paulista de Medicina, Universidade Federal de 
      Sao Paulo, Sao Paulo, Brazil.
FAU - Faca, Vitor Marcel
AU  - Faca VM
FAU - Gomes, Glauce Gaspar
AU  - Gomes GG
FAU - Chammas, Roger
AU  - Chammas R
FAU - Fontes, Aparecida Maria
AU  - Fontes AM
FAU - Covas, Dimas Tadeu
AU  - Covas DT
FAU - Greene, Lewis Joel
AU  - Greene LJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Proteomics
JT  - Proteomics
JID - 101092707
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Proteins)
RN  - 0 (Proteome)
RN  - 207137-56-2 (Interleukin-4)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Cell Differentiation/*physiology
MH  - Dendritic Cells/cytology/*drug effects/*physiology
MH  - Gene Expression Profiling
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/*pharmacology
MH  - Humans
MH  - Interleukin-4/*pharmacology
MH  - Lipopolysaccharides/*pharmacology
MH  - Molecular Sequence Data
MH  - Monocytes/physiology
MH  - Protein Processing, Post-Translational
MH  - Proteins/analysis
MH  - Proteome/*analysis
EDAT- 2005/04/01 09:00
MHDA- 2005/10/01 09:00
CRDT- 2005/04/01 09:00
PHST- 2005/04/01 09:00 [pubmed]
PHST- 2005/10/01 09:00 [medline]
PHST- 2005/04/01 09:00 [entrez]
AID - 10.1002/pmic.200400988 [doi]
PST - ppublish
SO  - Proteomics. 2005 Apr;5(5):1186-98. doi: 10.1002/pmic.200400988.