PMID- 15802502
OWN - NLM
STAT- MEDLINE
DCOM- 20050721
LR  - 20071115
IS  - 0013-7227 (Print)
IS  - 0013-7227 (Linking)
VI  - 146
IP  - 7
DP  - 2005 Jul
TI  - Growth hormone modulates thymocyte development in vivo through a combined action 
      of laminin and CXC chemokine ligand 12.
PG  - 3005-17
AB  - Previous evidence indicates that GH modulates thymic cell migration. In this 
      study we approached this issue in vivo, studying thymocyte migration in GH 
      transgenic animals and in normal mice treated intrathymically with GH. 
      Extracellular matrix and chemokines are involved in thymocyte migration. In this 
      respect, thymocyte adhesion to laminin was higher in GH-treated animals than 
      controls, and the numbers of migrating cells in laminin-coated Transwells was 
      higher in GH-transgenic and GH-injected mice. Additionally, CXC chemokine ligand 
      12 (CXCL12)-driven migration was higher in GH-Tg and GH-treated animals compared 
      with controls. Interestingly, although CXCR4 expression on thymocytes did not 
      change in GH-Tg mice, the CXCL12 intrathymic contents were higher. We found that 
      CXCL12, in conjunction with laminin, would additionally enhance the migration of 
      thymocytes previously exposed to high concentrations of GH in vivo. Lastly, there 
      was an augmentation of recent thymic emigrants in lymph nodes from GH-Tg and 
      GH-injected animals. In conclusion, enhanced thymocyte migration in GH transgenic 
      mice as well as GH-injected mice results at least partially from a combined 
      action of laminin and CXCL12. Considering that GH is presently being used as an 
      adjuvant therapeutic agent in immunodeficiencies, including AIDS, the concepts 
      defined herein provide important background knowledge for future GH-based immune 
      interventions.
FAU - Smaniotto, Salete
AU  - Smaniotto S
AD  - Institut National de la Sante et de la Recherche Medicale/Fiocruz Associate 
      Laboratory of Immunology, Laboratory on Thymus Research, Oswaldo Cruz Institute, 
      21045-900 Rio de Janeiro, Brazil.
FAU - de Mello-Coelho, Valeria
AU  - de Mello-Coelho V
FAU - Villa-Verde, Dea Maria Serra
AU  - Villa-Verde DM
FAU - Pleau, Jean-Marie
AU  - Pleau JM
FAU - Postel-Vinay, Marie-Catherine
AU  - Postel-Vinay MC
FAU - Dardenne, Mireille
AU  - Dardenne M
FAU - Savino, Wilson
AU  - Savino W
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050331
PL  - United States
TA  - Endocrinology
JT  - Endocrinology
JID - 0375040
RN  - 0 (Chemokine CXCL12)
RN  - 0 (Chemokines, CXC)
RN  - 0 (Cxcl12 protein, mouse)
RN  - 0 (Laminin)
RN  - 0 (Ligands)
RN  - 0 (Receptors, Cell Surface)
RN  - 0 (Recombinant Proteins)
RN  - 9002-72-6 (Growth Hormone)
SB  - IM
MH  - Animals
MH  - Animals, Genetically Modified
MH  - Cattle
MH  - Cell Adhesion/drug effects
MH  - Cell Movement/drug effects
MH  - Chemokine CXCL12
MH  - Chemokines, CXC/*pharmacology
MH  - Drug Synergism
MH  - Extracellular Matrix/metabolism
MH  - Female
MH  - Growth Hormone/administration & dosage/genetics/*pharmacology
MH  - Injections
MH  - Laminin/*pharmacology
MH  - Ligands
MH  - Lymph Nodes/cytology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Receptors, Cell Surface/metabolism
MH  - Recombinant Proteins/administration & dosage/pharmacology
MH  - Thymus Gland/*cytology/drug effects/*physiology
EDAT- 2005/04/02 09:00
MHDA- 2005/07/22 09:00
CRDT- 2005/04/02 09:00
PHST- 2005/04/02 09:00 [pubmed]
PHST- 2005/07/22 09:00 [medline]
PHST- 2005/04/02 09:00 [entrez]
AID - en.2004-0709 [pii]
AID - 10.1210/en.2004-0709 [doi]
PST - ppublish
SO  - Endocrinology. 2005 Jul;146(7):3005-17. doi: 10.1210/en.2004-0709. Epub 2005 Mar 
      31.