PMID- 15802508
OWN - NLM
STAT- MEDLINE
DCOM- 20051122
LR  - 20220409
IS  - 0016-6731 (Print)
IS  - 0016-6731 (Linking)
VI  - 170
IP  - 2
DP  - 2005 Jun
TI  - Chromosome sorting in tetraploid wheat and its potential for genome analysis.
PG  - 823-9
AB  - This study evaluates the potential of flow cytometry for chromosome sorting in 
      durum wheat (Triticum turgidum Desf. var. durum, 2n = 4x = 28). Histograms of 
      fluorescence intensity (flow karyotypes) obtained after the analysis of 
      DAPI-stained chromosomes consisted of three peaks. Of these, one represented 
      chromosome 3B, a small peak corresponded to chromosomes 1A and 6A, and a large 
      peak represented the remaining 11 chromosomes. Chromosomes sorted onto microscope 
      slides were identified after fluorescence in situ hybridization (FISH) with 
      probes for GAA microsatellite, pSc119.2, and Afa repeats. Genomic distribution of 
      these sequences was determined for the first time in durum wheat and a molecular 
      karyotype has been developed for this crop. Flow karyotyping in 
      double-ditelosomic lines of durum wheat revealed that the lines facilitated 
      sorting of any arm of the wheat A- and B-genome chromosomes. Compared to 
      hexaploid wheat, flow karyotype of durum wheat is less complex. This property 
      results in better discrimination of telosomes and high purities in sorted 
      fractions, ranging from 90 to 98%. We have demonstrated that large insert 
      libraries can be created from DNA purified using flow cytometry. This study 
      considerably expands the potential of flow cytogenetics for use in wheat genomics 
      and opens the possibility of sequencing the genome of this important crop one 
      chromosome arm at a time.
FAU - Kubalakova, Marie
AU  - Kubalakova M
AD  - Laboratory of Molecular Cytogenetics and Cytometry, Institute of Experimental 
      Botany, Olomouc, Czech Republic.
FAU - Kovarova, Pavlina
AU  - Kovarova P
FAU - Suchankova, Pavla
AU  - Suchankova P
FAU - Cihalikova, Jarmila
AU  - Cihalikova J
FAU - Bartos, Jan
AU  - Bartos J
FAU - Lucretti, Sergio
AU  - Lucretti S
FAU - Watanabe, Nobuyoshi
AU  - Watanabe N
FAU - Kianian, Shahryar F
AU  - Kianian SF
FAU - Dolezel, Jaroslav
AU  - Dolezel J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050331
PL  - United States
TA  - Genetics
JT  - Genetics
JID - 0374636
RN  - 0 (DNA, Plant)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Cell Cycle
MH  - Cell Separation
MH  - Chromosome Mapping
MH  - Chromosomes/ultrastructure
MH  - Chromosomes, Plant
MH  - DNA/genetics
MH  - DNA, Plant
MH  - Flow Cytometry
MH  - Genetic Techniques
MH  - Genome
MH  - *Genome, Plant
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotyping
MH  - Metaphase
MH  - Microsatellite Repeats
MH  - Models, Genetic
MH  - Physical Chromosome Mapping
MH  - Ploidies
MH  - Triticum/*genetics
PMC - PMC1450420
EDAT- 2005/04/02 09:00
MHDA- 2005/12/13 09:00
PMCR- 2005/09/01
CRDT- 2005/04/02 09:00
PHST- 2005/04/02 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/04/02 09:00 [entrez]
PHST- 2005/09/01 00:00 [pmc-release]
AID - genetics.104.039180 [pii]
AID - 9180 [pii]
AID - 10.1534/genetics.104.039180 [doi]
PST - ppublish
SO  - Genetics. 2005 Jun;170(2):823-9. doi: 10.1534/genetics.104.039180. Epub 2005 Mar 
      31.