PMID- 15802516
OWN - NLM
STAT- MEDLINE
DCOM- 20051122
LR  - 20181113
IS  - 0016-6731 (Print)
IS  - 0016-6731 (Linking)
VI  - 170
IP  - 2
DP  - 2005 Jun
TI  - The Bombyx mori karyotype and the assignment of linkage groups.
PG  - 675-85
AB  - Lepidopteran species have a relatively high number of small holocentric 
      chromosomes (Bombyx mori, 2n = 56). Chromosome identification has long been 
      hampered in this group by the high number and by the absence of suitable markers 
      like centromere position and chromosome bands. In this study, we carried out 
      fluorescence in situ hybridization (FISH) on meiotic chromosome complements using 
      genetically mapped B. mori bacterial artificial chromosomes (BACs) as probes. The 
      combination of two to four either green or red fluorescence-labeled probes per 
      chromosome allowed us to recognize unequivocally each of the 28 bivalents of the 
      B. mori karyotype by its labeling pattern. Each chromosome was assigned one of 
      the already established genetic linkage groups and the correct orientation in the 
      chromosome was defined. This facilitates physical mapping of any other sequence 
      and bears relevance for the ongoing B. mori genome projects. Two-color BAC-FISH 
      karyotyping overcomes the problem of chromosome recognition in organisms where 
      conventional banding techniques are not available.
FAU - Yoshido, Atsuo
AU  - Yoshido A
AD  - Division of Applied Bioscience, Graduate School of Agriculture, Hokkaido 
      University, Sapporo, Japan.
FAU - Bando, Hisanori
AU  - Bando H
FAU - Yasukochi, Yuji
AU  - Yasukochi Y
FAU - Sahara, Ken
AU  - Sahara K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20050331
PL  - United States
TA  - Genetics
JT  - Genetics
JID - 0374636
RN  - 0 (DNA Primers)
SB  - IM
MH  - Animals
MH  - Bombyx/*genetics/physiology
MH  - Chromosome Mapping
MH  - *Chromosomes, Artificial, Bacterial
MH  - DNA Primers/chemistry
MH  - *Genetic Linkage
MH  - Genetic Techniques
MH  - Image Processing, Computer-Assisted
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotyping
MH  - Models, Genetic
MH  - Random Amplified Polymorphic DNA Technique/methods
PMC - PMC1450397
EDAT- 2005/04/02 09:00
MHDA- 2005/12/13 09:00
PMCR- 2005/09/01
CRDT- 2005/04/02 09:00
PHST- 2005/04/02 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/04/02 09:00 [entrez]
PHST- 2005/09/01 00:00 [pmc-release]
AID - genetics.104.040352 [pii]
AID - 352 [pii]
AID - 10.1534/genetics.104.040352 [doi]
PST - ppublish
SO  - Genetics. 2005 Jun;170(2):675-85. doi: 10.1534/genetics.104.040352. Epub 2005 Mar 
      31.