PMID- 15807106
OWN - NLM
STAT- MEDLINE
DCOM- 20050913
LR  - 20220408
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 22
IP  - 6
DP  - 2004 Nov
TI  - [Determination of bisphenol A and 4-nonylphenol in rat tissues by high 
      performance liquid chromatography with fluorescence detection].
PG  - 579-82
AB  - A simple and highly sensitive method has been developed for the determination of 
      bisphenol A (BPA) and 4-nonylphenol (4-NP) in rat tissues by high performance 
      liquid chromatography (HPLC) with fluorescence detection. The rat tissue samples 
      were homogenized-with methanol-ammonium acetate buffer (2:8, v/v) by a high-speed 
      homogenizer and then BPA and 4-NP were extracted by a mixed solvent of n-hexane 
      and diethyl ether (7:3, v/v). The organic layer was evaporated with a stream of 
      nitrogen, and the residue was dissolved in the mobile phase. The chromatographic 
      operating conditions were a C18 column (250 mm x 4. 6 mm i.d., 5 microm), 
      acetonitrile-ammonium acetate buffer (pH 4.5) (75:25, v/v) as mobile phase at a 
      flow rate of 1 mL/min, fluorescence detection with the excitation and emission 
      wavelengths at 227 nm and 313 nm, respectively. Average recoveries for rat 
      tissues at three different levels were in the range of 82.0% -95.4% for BPA and 
      81.2% -96.5% for 4-NP. The relative standard deviations (RSDs) were 0. 37% - 
      5.28% and the detection limits were 4.0, 4.6, 3.2, 3.3 ng/g for BPA and 12.0, 
      15.6, 11.8, 13.8 ng/g for 4-NP in liver, kidney, heart and brain tissues, 
      respectively. The intra-day precisions were 0.89% -4.50%, and the inter-day 
      precisions were 3.10% - 12.40%. These results demonstrated that the proposed 
      method is simple, sensitive, and reliable for the determination of BPA and 4-NP 
      in animal tissues.
FAU - Xiao, Quanwei
AU  - Xiao Q
AD  - West China School of Public Health, Sichuan University, Chengdu 610041, China.
FAU - Li, Yuanqian
AU  - Li Y
FAU - Wu, Desheng
AU  - Wu D
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Benzhydryl Compounds)
RN  - 0 (Phenols)
RN  - I03GBV4WEL (4-nonylphenol)
RN  - MLT3645I99 (bisphenol A)
SB  - IM
MH  - Animals
MH  - Benzhydryl Compounds
MH  - *Brain Chemistry
MH  - Chromatography, High Pressure Liquid
MH  - Female
MH  - Phenols/*analysis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Spectrometry, Fluorescence/methods
EDAT- 2005/04/06 09:00
MHDA- 2005/09/15 09:00
CRDT- 2005/04/06 09:00
PHST- 2005/04/06 09:00 [pubmed]
PHST- 2005/09/15 09:00 [medline]
PHST- 2005/04/06 09:00 [entrez]
PST - ppublish
SO  - Se Pu. 2004 Nov;22(6):579-82.