PMID- 15807112
OWN - NLM
STAT- MEDLINE
DCOM- 20050913
LR  - 20161018
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 22
IP  - 6
DP  - 2004 Nov
TI  - [Determination of curcumin in urine by capillary electrophoresis].
PG  - 609-12
AB  - The major component of the plant curcuma longa (a widely cultivated tropical 
      plant in Asia and Central America) is curcumin. Curcumin has been reported to 
      have very strong anti-inflammatory, anti-carcinogenic, anti-oxidant, 
      antiallergic, anti-bacterial, and anti-tumor activities. Little is known about 
      the absorption, distribution, and metabolism of curcumin in human beings. The 
      first step in in vivo physiological and pharmacokinetic studies is to develop a 
      method to measure curcumin in body fluid. A rapid capillary electrophoretic (CE) 
      method with diode array detection was established for the determination of 
      curcumin in human urine. It could be rapidly determined within 2.5 min. The 
      optimized experimental conditions were as follows: 15 mmol/L Na2B4O7 as buffer, 
      applied voltage 20 kV, temperature 25 degrees C and detection wavelength 262 nm. 
      The method has been validated and shows good performance with respect to 
      selectivity, reproducibility and limit of detection. Curcumin had good linearity 
      in the range of 10 - 300 mg/L, and the recoveries of curcumin added in urine were 
      more than 96.3% with relative standard deviations (RSDs) less than 2.3%. The 
      method is sensitive, fast and accurate and can be used to determine curcumin in 
      urine.
FAU - Yuan, Kailong
AU  - Yuan K
AD  - National Chromatographic R. & A. Center, Dalian Institute of Chemical Physics, 
      The Chinese Academy of Sciences, Dalian 116011, China.
FAU - Weng, Qianfeng
AU  - Weng Q
FAU - Zhang, Hongying
AU  - Zhang H
FAU - Xiong, Jianhui
AU  - Xiong J
FAU - Yang, Jun
AU  - Yang J
FAU - Xu, Guowang
AU  - Xu G
LA  - chi
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Drugs, Chinese Herbal)
RN  - IT942ZTH98 (Curcumin)
SB  - IM
MH  - Curcuma/chemistry
MH  - Curcumin/*analysis
MH  - Drugs, Chinese Herbal/chemistry
MH  - *Electrophoresis, Capillary
MH  - Humans
MH  - Sensitivity and Specificity
EDAT- 2005/04/06 09:00
MHDA- 2005/09/15 09:00
CRDT- 2005/04/06 09:00
PHST- 2005/04/06 09:00 [pubmed]
PHST- 2005/09/15 09:00 [medline]
PHST- 2005/04/06 09:00 [entrez]
PST - ppublish
SO  - Se Pu. 2004 Nov;22(6):609-12.