PMID- 15807616
OWN - NLM
STAT- MEDLINE
DCOM- 20060808
LR  - 20061115
IS  - 0743-7463 (Print)
IS  - 0743-7463 (Linking)
VI  - 21
IP  - 8
DP  - 2005 Apr 12
TI  - Cast thin film biosensor design based on a Nafion backbone, a multiwalled carbon 
      nanotube conduit, and a glucose oxidase function.
PG  - 3653-8
AB  - Novel electroanalytical sensing nanobiocomposite materials are reported. These 
      materials are prepared by mixing multiwalled carbon nanotubes (MWNTs), a Nafion 
      cation exchanger, and glucose oxidase (GOD) in appropriate amounts. The MWNTs are 
      cylindrical with a diameter in the range 40-60 nm and with a length of up to 
      several micrometers, and they provide electrical conductivity. Nafion acts as a 
      polymer backbone to give stable and homogeneous cast thin films. Both MWNTs and 
      Nafion provide negative functionalities to bind to positively charged redox 
      enzymes such as glucose oxidase. The resulting biosensing composite material is 
      inexpensive, reliable, and easy to use. The homogeneity of the MWNT-Nafion-GOD 
      nanobiocomposite films was characterized by atomic force microscopy (AFM). 
      Amperometric transducers fabricated with these materials were characterized 
      electrochemically using cyclic voltammetry and amperometry in the presence of 
      hydrogen peroxide and in the presence of glucose. Their linear response to 
      hydrogen peroxide was demonstrated. The glucose biosensor sensitivity was 
      strongly influenced by the glucose oxidase concentration within the 
      nanobiocomposite film. The optimized glucose biosensor (2.5 mg/mL GOD) displayed 
      a sensitivity of 330 nA/mM, a linear range of up to 2 mM, a detection limit of 4 
      microM, and a response time of <3 s.
FAU - Tsai, Yu-Chen
AU  - Tsai YC
AD  - Department of Chemical Engineering, National Chung Hsing University, Taichung 
      402, Taiwan. yctsai@dragon.nchu.edu.tw
FAU - Li, Shih-Ci
AU  - Li SC
FAU - Chen, Jie-Ming
AU  - Chen JM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Langmuir
JT  - Langmuir : the ACS journal of surfaces and colloids
JID - 9882736
RN  - 0 (Fluorocarbon Polymers)
RN  - 0 (Nanotubes, Carbon)
RN  - 39464-59-0 (perfluorosulfonic acid)
RN  - EC 1.1.3.4 (Glucose Oxidase)
SB  - IM
MH  - Biosensing Techniques/*methods
MH  - Electrochemistry
MH  - Fluorocarbon Polymers/*chemistry
MH  - Glucose Oxidase/*chemistry
MH  - Nanotechnology/*methods
MH  - Nanotubes, Carbon/*chemistry
MH  - Sensitivity and Specificity
MH  - Spectroscopy, Fourier Transform Infrared
EDAT- 2005/04/06 09:00
MHDA- 2006/08/09 09:00
CRDT- 2005/04/06 09:00
PHST- 2005/04/06 09:00 [pubmed]
PHST- 2006/08/09 09:00 [medline]
PHST- 2005/04/06 09:00 [entrez]
AID - 10.1021/la0470535 [doi]
PST - ppublish
SO  - Langmuir. 2005 Apr 12;21(8):3653-8. doi: 10.1021/la0470535.