PMID- 15807855
OWN - NLM
STAT- MEDLINE
DCOM- 20050509
LR  - 20240413
IS  - 0009-9104 (Print)
IS  - 1365-2249 (Electronic)
IS  - 0009-9104 (Linking)
VI  - 140
IP  - 2
DP  - 2005 May
TI  - Different natural killer (NK) receptor expression and immunoglobulin E (IgE) 
      regulation by NK1 and NK2 cells.
PG  - 301-9
AB  - Many studies concerning the role of T cells and cytokines in allergy have been 
      performed, but little is known about the role of natural killer (NK) cells. 
      Accordingly, the expression of co-stimulatory, inhibitory and apoptosis 
      receptors, cytokine profiles and their effect on immunoglobulin isotypes were 
      investigated in polyallergic atopic dermatitis (AD) patients with hyper 
      immunoglobulin E (IgE) and healthy individuals. AD patients showed significantly 
      decreased peripheral blood NK cells compared to healthy individuals. Freshly 
      isolated NK cells of polyallergic patients spontaneously released higher amounts 
      of interleukin (IL)-4, IL-5, IL-13 and interferon (IFN)-gamma compared to healthy 
      individuals. NK cells were differentiated to NK1 cells by IL-12 and neutralizing 
      anti-IL-4 monoclonal antibodies (mAb), and to NK2 cells by IL-4 and neutralizing 
      anti-IL-12 mAb. Following IL-12 stimulation, NK cells produced increased levels 
      of IFN-gamma and decreased IL-4. In contrast, stimulation of NK cells with IL-4 
      inhibited IFN-gamma, but increased IL-13, production. The effect of NK cell 
      subsets on IgE regulation was examined in co-cultures of in vitro differentiated 
      NK cells with peripheral blood mononuclear cells (PBMC) or B cells. NK1 cells 
      significantly inhibited IL-4- and soluble CD40-ligand-stimulated IgE production; 
      however, NK2 cells did not have any effect. The inhibitory effect of NK1 cells on 
      IgE production was blocked by neutralization of IFN-gamma. Except for CD40, NK 
      cell subsets showed different expression of killer-inhibitory receptors and 
      co-stimulatory molecules between the polyallergic and healthy subjects. These 
      results indicate that human NK cells show differences in numbers, surface 
      receptor and cytokine phenotypes and functional properties in AD.
FAU - Aktas, E
AU  - Aktas E
AD  - Istanbul University, Institute of Experimental Medical Research (DETAE), 
      Istanbul, Turkey.
FAU - Akdis, M
AU  - Akdis M
FAU - Bilgic, S
AU  - Bilgic S
FAU - Disch, R
AU  - Disch R
FAU - Falk, C S
AU  - Falk CS
FAU - Blaser, K
AU  - Blaser K
FAU - Akdis, C
AU  - Akdis C
FAU - Deniz, G
AU  - Deniz G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Clin Exp Immunol
JT  - Clinical and experimental immunology
JID - 0057202
RN  - 0 (Cytokines)
RN  - 0 (Receptors, Immunologic)
RN  - 0 (Receptors, KIR)
RN  - 187348-17-0 (Interleukin-12)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adult
MH  - Apoptosis/immunology
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Cytokines/biosynthesis
MH  - Dermatitis, Atopic/*immunology
MH  - Humans
MH  - Immunoglobulin E/*biosynthesis
MH  - Interferon-gamma/biosynthesis/immunology
MH  - Interleukin-12/immunology
MH  - Interleukin-4/biosynthesis
MH  - Killer Cells, Natural/*immunology
MH  - Leukocytes, Mononuclear/immunology
MH  - Receptors, Immunologic/*metabolism
MH  - Receptors, KIR
PMC - PMC1809360
EDAT- 2005/04/06 09:00
MHDA- 2005/05/10 09:00
PMCR- 2006/05/01
CRDT- 2005/04/06 09:00
PHST- 2005/04/06 09:00 [pubmed]
PHST- 2005/05/10 09:00 [medline]
PHST- 2005/04/06 09:00 [entrez]
PHST- 2006/05/01 00:00 [pmc-release]
AID - CEI2777 [pii]
AID - 10.1111/j.1365-2249.2005.02777.x [doi]
PST - ppublish
SO  - Clin Exp Immunol. 2005 May;140(2):301-9. doi: 10.1111/j.1365-2249.2005.02777.x.