PMID- 15808072
OWN - NLM
STAT- MEDLINE
DCOM- 20051122
LR  - 20161124
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 85
IP  - 1
DP  - 2005 Jan 5
TI  - [Exogenous PTEN gene induces apoptosis in breast cancer cells].
PG  - 33-6
AB  - OBJECTIVE: To investigate the effects of exogenous PTEN gene on apoptosis of 
      breast cancer cells. METHODS: Human breast cancer cells of the line MDA468 were 
      cultured. Recombinant plasmid pcDNA3.1-PTEN was constructed and transfected into 
      the breast cancer cells with the lipofectAMINE 2000 transfection technique. 
      Parental MDA468 cells and parental MDA468 cells transfected with blank vector 
      pcDNA3.1(-) were used as control groups. RT-PCR was used to detect the expression 
      of the PTEN mRNA and Western blotting was used to determine the expression of 
      PTEN protein. Epithelial growth factor (EGF) was added into the cultures of 
      MDA468 cells transfected with pcDNA3.1-PTEN or blank vector. Then Western 
      blotting was used to detect the expression of phosphospecific protein kinase B 
      (PKB/Akt) and focal adhesion kinase (FAK) protein stimulated by EGF. The 
      aapoptosis of the MDA468 cells was determined by flow cytometry with the 
      double-staining method using FITC-conjugated annexin V and PI. RESULTS: 
      Expressions of PTEN mRNA and protein were shown in the MDA468 cells transfected 
      with pcDNA3.1-PTEN by RT-PCR and Western blotting. Both the expression of p-AKT 
      and that of p-FAK were down-regulated in the MDA468 cells transfected with 
      pcDNA3.1-PTEN in comparison with those in the control cells. The apoptotic rate 
      of the MDA468 cells transfected with PCDNA3.1-PTEN was 21.68%, significantly 
      higher than those of the blank control cells (1.17%) and pcDNA3.1(-)-transfected 
      cells (3.55%, both P < 0.01). CONCLUSION: Exogenous PTEN suppress the growth of 
      human breast cancer cell and induces apoptosis by phosphatase activity, which 
      provides a new clue to gene therapy for breast cancer.
FAU - Chen, Qing-Yong
AU  - Chen QY
AD  - Department of Emergency Surgery, Xiehe Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan 430022, China.
FAU - Wang, Chun-You
AU  - Wang CY
FAU - Wu, He-Shui
AU  - Wu HS
FAU - Chen, Dao-da
AU  - Chen DD
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (RNA, Messenger)
RN  - EC 3.1.3.67 (PTEN Phosphohydrolase)
RN  - EC 3.1.3.67 (PTEN protein, human)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Breast Neoplasms/*pathology
MH  - Cell Line, Tumor
MH  - Female
MH  - Genes, Tumor Suppressor
MH  - Genetic Therapy
MH  - Humans
MH  - PTEN Phosphohydrolase/biosynthesis/genetics/*pharmacology
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Transfection
EDAT- 2005/04/06 09:00
MHDA- 2005/12/13 09:00
CRDT- 2005/04/06 09:00
PHST- 2005/04/06 09:00 [pubmed]
PHST- 2005/12/13 09:00 [medline]
PHST- 2005/04/06 09:00 [entrez]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2005 Jan 5;85(1):33-6.