PMID- 15808527
OWN - NLM
STAT- MEDLINE
DCOM- 20051028
LR  - 20050405
IS  - 0041-1345 (Print)
IS  - 0041-1345 (Linking)
VI  - 37
IP  - 1
DP  - 2005 Jan-Feb
TI  - The effects of renal transplantation on circulating precursor dendritic cells.
PG  - 3-6
AB  - BACKGROUND: Dendritic cells (DCs) are potent antigen-presenting cells that induce 
      and regulate immune responses. Recent advances allow accurate quantification of 
      peripheral blood (PB) myeloid and plasmacytoid DC populations (mDC and pDC, 
      respectively), although the response to renal transplantation (RT) remains 
      unknown. METHODS: Using flow cytometry, PBDC levels were quantified in patients 
      with end-stage renal disease (ESRD) undergoing renal transplantation. RESULTS: 
      PBDC levels were significantly reduced in ESRD patients pretransplantation 
      compared to healthy controls, with further reduction noted immediately following 
      a hemodialysis session. RT resulted in a dramatic decrease in both subsets, with 
      a greater reduction of pDC levels. Both subset levels were significantly lower 
      than in control patients undergoing abdominal surgery without RT. Subgroup 
      analysis revealed significantly greater mDC reduction in RT recipients receiving 
      antilymphocyte therapy, with preferential binding of antibody preparation to this 
      subset. Samples from later time points revealed a gradual return of PBDC levels 
      back to pretransplant values concurrent with overall reduction of 
      immunosuppression. Finally, PBDC levels were significantly reduced in patients 
      with BK virus nephropathy compared to recipients with stable graft function, 
      despite lower overall immunosuppression. CONCLUSIONS: Our findings suggest that 
      PBDC levels may reflect the degree of immunosuppression in renal allograft 
      recipients. Furthermore, PBDC monitoring may represent a novel strategy to 
      predict important outcomes such as acute rejection, long-term graft loss, and 
      infectious complications.
FAU - Womer, K L
AU  - Womer KL
AD  - Department of Medicine, Gainesville, Florida, USA. womerkl@ufl.edu
FAU - Peng, R
AU  - Peng R
FAU - Patton, P R
AU  - Patton PR
FAU - Kaleem, A
AU  - Kaleem A
FAU - Bucci, M
AU  - Bucci M
FAU - Murawski, M R
AU  - Murawski MR
FAU - Schold, J
AU  - Schold J
FAU - Efron, P A
AU  - Efron PA
FAU - Hemming, A W
AU  - Hemming AW
FAU - Srinivas, T R
AU  - Srinivas TR
FAU - Meier-Kriesche, H-U
AU  - Meier-Kriesche HU
FAU - Kaplan, B
AU  - Kaplan B
FAU - Clare-Salzler, M J
AU  - Clare-Salzler MJ
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Transplant Proc
JT  - Transplantation proceedings
JID - 0243532
SB  - IM
MH  - Adult
MH  - Dendritic Cells/*immunology
MH  - Female
MH  - Flow Cytometry
MH  - Humans
MH  - Kidney Failure, Chronic/immunology/surgery
MH  - Kidney Transplantation/*immunology
MH  - Male
MH  - Middle Aged
MH  - Reference Values
MH  - Stem Cells/immunology
EDAT- 2005/04/06 09:00
MHDA- 2005/10/29 09:00
CRDT- 2005/04/06 09:00
PHST- 2005/04/06 09:00 [pubmed]
PHST- 2005/10/29 09:00 [medline]
PHST- 2005/04/06 09:00 [entrez]
AID - S0041-1345(04)01387-9 [pii]
AID - 10.1016/j.transproceed.2004.11.076 [doi]
PST - ppublish
SO  - Transplant Proc. 2005 Jan-Feb;37(1):3-6. doi: 10.1016/j.transproceed.2004.11.076.