PMID- 15808617
OWN - NLM
STAT- MEDLINE
DCOM- 20051028
LR  - 20061115
IS  - 0041-1345 (Print)
IS  - 0041-1345 (Linking)
VI  - 37
IP  - 1
DP  - 2005 Jan-Feb
TI  - Can a bone marrow cell contribute to organ regeneration? In vivo analysis using 
      transgenic rats with reporter genes.
PG  - 273-5
AB  - Although implantation of multipotent bone marrow-derived stem cells represents an 
      attractive new cell therapy to repair damaged tissues, recent reports have raised 
      serious concerns over the feasibility of using stem cells deriving from the bone 
      marrow to promote cell transdifferentiation. We established transgenic (Tg) rats 
      with reporter genes as specific molecular tags to examine the effect of bone 
      marrow cells (BMCs) on transdifferentiation into tissues/organs. To monitor 
      transdifferentiation events of locally transplanted BMCs into hepatocytes or 
      capillary endothelial cells, a liver injury model and an ischemic hind-limb model 
      were developed in rats. To test the ability of circulating bone marrow-derived 
      cells to give rise to myocytes after skeletal muscle injury, we used a bone 
      marrow cell transplantation model from Tg rats, which showed ubiquitous 
      expression of beta-galactosidase (lacZ), into lethally irradiated non-Tg rats. 
      Our results show that there was little transdifferentiation of BMCs into the 
      targeted cells in these tissue injury models. However, in the ischemic hind-limb 
      model, laser Doppler imaging and histologic analysis showed that both 
      implantation of BMCs and treatment with microspheres incorporating basic 
      fibroblast-like growth factor (bFGF), which enables the release of bFGF at the 
      site of action over a period of time, effectively induced angiogenesis. In 
      conclusion, rat BMCs with specific marker genes could be a useful tool for 
      detecting transdifferentiation events in vivo.
FAU - Sato, Y
AU  - Sato Y
AD  - Division of Organ Replacement Research, Center for Molecular Medicine, Jichi 
      Medical School, Tochigi, Japan.
FAU - Matsui, K
AU  - Matsui K
FAU - Ajiki, T
AU  - Ajiki T
FAU - Igarashi, Y
AU  - Igarashi Y
FAU - Takahashi, M
AU  - Takahashi M
FAU - Murakami, T
AU  - Murakami T
FAU - Hakamata, Y
AU  - Hakamata Y
FAU - Tabata, Y
AU  - Tabata Y
FAU - Kobayashi, E
AU  - Kobayashi E
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Transplant Proc
JT  - Transplantation proceedings
JID - 0243532
RN  - EC 3.2.1.23 (beta-Galactosidase)
SB  - IM
MH  - Animals
MH  - Animals, Genetically Modified
MH  - Bone Marrow Cells/*cytology
MH  - Genes, Reporter
MH  - Hepatocytes/*transplantation
MH  - Hindlimb/blood supply
MH  - Ischemia
MH  - Rats
MH  - Regeneration
MH  - Stem Cell Transplantation/*methods
MH  - beta-Galactosidase/genetics
EDAT- 2005/04/06 09:00
MHDA- 2005/10/29 09:00
CRDT- 2005/04/06 09:00
PHST- 2005/04/06 09:00 [pubmed]
PHST- 2005/10/29 09:00 [medline]
PHST- 2005/04/06 09:00 [entrez]
AID - S0041-1345(05)00042-4 [pii]
AID - 10.1016/j.transproceed.2005.01.035 [doi]
PST - ppublish
SO  - Transplant Proc. 2005 Jan-Feb;37(1):273-5. doi: 
      10.1016/j.transproceed.2005.01.035.