PMID- 15811707
OWN - NLM
STAT- MEDLINE
DCOM- 20050721
LR  - 20220408
IS  - 0378-5955 (Print)
IS  - 0378-5955 (Linking)
VI  - 202
IP  - 1-2
DP  - 2005 Apr
TI  - Dexamethasone inhibits tumor necrosis factor-alpha-induced cytokine secretion 
      from spiral ligament fibrocytes.
PG  - 154-60
AB  - To investigate the effect of proinflammatory cytokines on spiral ligament (SL) 
      fibrocytes and regulation of cytokines by dexamethasone (Dex), in vitro studies 
      were performed in murine secondary cell cultures. Cultured SL fibrocytes were 
      stimulated with tumor necrosis factor-alpha (TNF-alpha), and the secretion of 
      various mediators was measured by enzyme-linked immunosorbent assay (ELISA) and 
      reverse transcribed-polymerase chain reaction (RT-PCR). After stimulation with 
      TNF-alpha, levels of keratinocyte-derived cytokine (KC), monocyte chemoattractant 
      protein-1 (MCP-1), macrophage inflammatory protein-2 (MIP-2), interleukin-6 
      (IL-6) and soluble intercellular adhesion molecule-1 (sICAM-1) were elevated in 
      the culture supernatant, and their corresponding messenger RNAs were detected in 
      the cultured fibrocytes. When the cultures were incubated with both TNF-alpha and 
      Dex, the levels of KC, MCP-1, MIP-2 and IL-6 were significantly lower than those 
      in cultures treated with TNF-alpha alone. The data suggest that Dex suppresses 
      the inflammatory response in SL fibrocytes. Given that SL fibrocytes play a role 
      in cochlear fluid and ion homeostasis, glucocorticoids may suppress the cochlear 
      malfunction caused by SL inflammation.
FAU - Maeda, Keiko
AU  - Maeda K
AD  - Department of Otolaryngology, Faculty of Medicine, Oita University, Hasama-machi, 
      Japan.
FAU - Yoshida, Kazuhide
AU  - Yoshida K
FAU - Ichimiya, Issei
AU  - Ichimiya I
FAU - Suzuki, Masashi
AU  - Suzuki M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Hear Res
JT  - Hearing research
JID - 7900445
RN  - 0 (Cytokines)
RN  - 0 (Glucocorticoids)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 7S5I7G3JQL (Dexamethasone)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Cytokines/*antagonists & inhibitors/genetics/metabolism
MH  - Dexamethasone/*pharmacology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Glucocorticoids/*pharmacology
MH  - Male
MH  - Mice
MH  - Mice, Inbred CBA
MH  - Organ of Corti/cytology/*metabolism
MH  - RNA, Messenger/antagonists & inhibitors/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 2005/04/07 09:00
MHDA- 2005/07/22 09:00
CRDT- 2005/04/07 09:00
PHST- 2004/07/21 00:00 [received]
PHST- 2004/08/11 00:00 [accepted]
PHST- 2005/04/07 09:00 [pubmed]
PHST- 2005/07/22 09:00 [medline]
PHST- 2005/04/07 09:00 [entrez]
AID - S0378-5955(04)00364-8 [pii]
AID - 10.1016/j.heares.2004.08.022 [doi]
PST - ppublish
SO  - Hear Res. 2005 Apr;202(1-2):154-60. doi: 10.1016/j.heares.2004.08.022.