PMID- 15812841
OWN - NLM
STAT- MEDLINE
DCOM- 20050816
LR  - 20131121
IS  - 0173-0835 (Print)
IS  - 0173-0835 (Linking)
VI  - 26
IP  - 9
DP  - 2005 May
TI  - A facile and sensitive chemiluminescence detection of amino acids in biological 
      samples after capillary electrophoretic separation.
PG  - 1745-50
AB  - It was found that native amino acids enhanced the chemiluminescence (CL) reaction 
      between luminol and BrO(-) in an alkaline aqueous solution. This has led to the 
      development of a facile and highly sensitive CL detection scheme for the 
      determination of amino acids in biological samples after capillary 
      electrophoretic (CE) separation. The CE-CL conditions were optimized. An 
      electrophoretic buffer of 2.5 x 10(-2) M sodium borate (pH 9.4) containing 1 x 
      10(-4) M luminol was used. The oxidizer solution of 8 x 10(-4) M NaBrO in 0.1 M 
      sodium carbonate buffer solution (pH 12.5) was introduced post-column. Under the 
      optimal conditions, the detection limits were 1.0 x 10(-7) M for glutamic acid 
      (Glu) and 1.3 x 10(-7) M (S/N = 3) for aspartic acid (Asp). The relative standard 
      deviations (RSDs) of peak area and migration time were in the ranges of 3.8-4.3% 
      and 1.4-1.6%, respectively. The present method was applied to the determination 
      of excitatory amino acids (i.e., Asp and Glu) in rat brain tissue and monkey 
      plasma. The levels of these major excitatory amino acids in monkey plasma were 
      quantified for the first time and found to be 1.17 +/- 0.17 x 10(-5) M (mean +/- 
      SD, n = 6) for Glu and 1.64 +/- 0.19 x 10(-6) M for Asp, which were comparable 
      with the levels in human plasma.
FAU - Zhao, Shulin
AU  - Zhao S
AD  - College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, 
      China.
FAU - Xie, Chao
AU  - Xie C
FAU - Lu, Xin
AU  - Lu X
FAU - Song, Yaru
AU  - Song Y
FAU - Liu, Yi-Ming
AU  - Liu YM
LA  - eng
GR  - NS 44177/NS/NINDS NIH HHS/United States
GR  - S06 GM 08047/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Germany
TA  - Electrophoresis
JT  - Electrophoresis
JID - 8204476
RN  - 0 (Excitatory Amino Acids)
RN  - 5EXP385Q4F (Luminol)
SB  - IM
MH  - Animals
MH  - Brain Chemistry
MH  - *Electrophoresis, Capillary
MH  - Excitatory Amino Acids/*analysis/blood/isolation & purification
MH  - Haplorhini
MH  - Luminescent Measurements/*methods
MH  - Luminol/chemistry
MH  - Rats
EDAT- 2005/04/07 09:00
MHDA- 2005/08/17 09:00
CRDT- 2005/04/07 09:00
PHST- 2005/04/07 09:00 [pubmed]
PHST- 2005/08/17 09:00 [medline]
PHST- 2005/04/07 09:00 [entrez]
AID - 10.1002/elps.200410338 [doi]
PST - ppublish
SO  - Electrophoresis. 2005 May;26(9):1745-50. doi: 10.1002/elps.200410338.